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Development of a method for the identification of novel viruses in marsupials with the polymerase chain reaction (PCR)

Four main types of viruses capable of causing systemic and gastrointestinal infections, namely Coronavirus, Rotavirus, Parvovirus or Morbillivirus (Tennant et al, 1991) have been investigated in marsupials. A pilot study to develop and optimise the methodology was undertaken using Canine Coronavirus and the study was then extended to marsupials and other target viruses.In the marsupial portion of the study, a fragment of the correct size for the amplification of pol gene, 409 bp, was obtained from two different faecal samples from tammar wallaby (from Macquarie Fauna Park) and one western grey kangaroo (from Taronga Zoo). The results from tests are studied and compared. It is suggested that future further investigation should be directed at: 1/. Applying the protocols in this body of work to the testing of faecal samples from animals that have been diagnosed as positive with other diagnostic protocols. 2/. Examining more faecal samples from animals that present with active diarrhoea / Master of Science (Hons)

Identiferoai:union.ndltd.org:ADTP/182431
Date January 2002
CreatorsWalia, Charanjiv Singh, University of Western Sydney, College of Science, Technology and Environment, School of Science, Food and Horticulture
Source SetsAustraliasian Digital Theses Program
LanguageEnglish
Detected LanguageEnglish
SourceTHESIS_CSTE_SFH_Walia_C.xml

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