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Characterisation of lipase genes in Helicoverpa armigera

Helicoverpa armigera (cotton bollworm) is a major agricultural pest in Australia, Asia and Africa. Analysis of a midgut cDNA library revealed a surprising diversity of genes encoding lipases, enzymes that metabolise lipids. Prior to and during this project, 13 neural lipases and three acidic ones were discovered. These were classified and compared to other insect lipases using sequence alignments and phylogenetic analysis. Novel non-catalytic subfamilies were discovered, including one containing an H. armigera lipase. At least three other subfamilies were found to contain H. armigera lipases. Lipidopteran lipases were found to be almost entirely distinct from Dipteran ones.

Identiferoai:union.ndltd.org:ADTP/245707
Date January 2006
CreatorsTan-Kristanto, Ariadne
Source SetsAustraliasian Digital Theses Program
LanguageEnglish
Detected LanguageEnglish
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