Includes bibliographical references. The aim of this work is the further characterisation of the citrus exocortis viroid (CEV) replication complex in a tomato host system. The study employed three approaches. i. To raise polyclonal antiserum against a putative catalytic component of the CEV replication complex, the second largest subunit of tomato RNA polymerase II--ii. To develop a cell-free system for CEV RNA synthesis--iii. To use the polyclonal antiserum to the second largest subunit of RNA polymerase II, and a commercially available monoclonal antibody to the largest subunit, for antibody inhibition and immunoprecipitation experiments using the cell-free system.
| Identifer | oai:union.ndltd.org:ADTP/65292 |
| Date | January 1996 |
| Creators | Warrilow, David, 1968- |
| Source Sets | Australiasian Digital Theses Program |
| Language | English |
| Detected Language | English |
| Relation | SUA, SUA:W |
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