Effects of homologous extracts prepared from mature avian skeletal muscle on the development of isolated myoblasts from the thigh muscle of 11-12 day old chick embryoswere studied in vitro under previously unpublished culture conditions. Results of these experiments indicate that:1) fusion of myoblasts can occur in a predictable manner under the culture conditions described in this report2) some factor(s) present in extracts of homologous adult organ is able to partially inhibit this fusion3) such inhibition occurs maximally between 12 and 24 hours after myoblasts are placed in an in vitro environment, and is only partially reversible by the re-establishment of optimal culture conditions4) the inhibitory factor(s) is apparently long lived, non-dialyzablQ, heat labile, and subject to inactivation by proteolytic enzymes.
| Identifer | oai:union.ndltd.org:BSU/oai:cardinalscholar.bsu.edu:handle/180661 |
| Date | January 1973 |
| Creators | Bishop, William E. |
| Contributors | Bennett, Alice S. |
| Source Sets | Ball State University |
| Detected Language | English |
| Format | iii, 50 leaves : ill. ; 28 cm. |
| Source | Virtual Press |
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