Benzylpenicilloyl-poly-L-cysteine is prepared by reacting benzylpenicillenic acid with poly-L-cysteine at 370° in water at pH 8.3 or buffer at pH 7.98. The preparations of penicilloyl-cysteine, S-acetamidomethyl-polycysteine, and S-acetamidomethyl-penicilloyl-polycysteine are also described. C14-labeling and penamaldate assays are used to determine penicilloyl content. The iodoacetic acid method is used to ascertain thiol content.The ultraviolet spectra for penicilloyl-polycysteine, pencilloyl-cysteine, poly-L-cysteine, poly-S-carbobenzoxy-L-cysteine, S-acetamidomethyl-polycysteine and S-acetamidomethyl-penicilloyl-polycysteine are reported. The infrared spectra of penicilloyl-polycysteine, penicilloyl-cysteine, poly-S-carbobenzoxy-L-cysteine, S-acetamidomethyl-polycysteine, and S-acetamidomethyl-penicilloyl-polycysteine are reported. The nmr spectra of poly-L-cysteine, penicilloyl-cysteine, S-acetamidomethyl-polycysteine, and S-acetamidomethyl-penicilloyl-polycysteine are discussed.The kinetic rates of reaction of benzylpenicillenic acid at 37.5 ± 0.50 in buffer, cysteine, N, S-di-CBZ L-cysteine, poly-L-cysteine, poly-S-CBZ L-cysteine andβ-mercaptoethylamine are compared.Ball State UniversityMuncie, IN 47306
| Identifer | oai:union.ndltd.org:BSU/oai:cardinalscholar.bsu.edu:handle/180898 |
| Date | 03 June 2011 |
| Creators | Storhoff, Diana F. |
| Contributors | Wagner, Eugene S. |
| Source Sets | Ball State University |
| Detected Language | English |
| Format | vi, 63 leaves : ill. ; 28 cm. |
| Source | Virtual Press |
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