This study was conducted to determine the variation in stain Intensity between serial sections of muscle biopsies following a periodic acid Schiff (PAS) staining procedure, to assess the reliability of the PAS staining technique for the quantitation of the glycogen content in muscle fibers, and to evaluate the variability in fiber composition between repeated biopsies of the vastus lateralis (VL) muscle. Eight randomly located biopsies (4 right leg and 4 left leg) were obtained from the VL of each of 16 healthy males (26.1 ± 1.1 years). Serial cross sections, 10 um thick, were cut from each biopsy and stained for myosin ATPase following an acid preincubation at pH=4.30 and for glycogen using a PAS staining procedure. No significant difference existed in the fiber composition between the eight repeated biopsies taken from an individual. The variation In type I fiber percentage, expressed as the coefficient of variation, between repeated biopsies of the same leg and between the right and left VL averaged 18.6% and 17.7%, respectively. In many cases, differences of greater than 20% In the percentage of type I fibers were observed between repeated samples. These data suggest an inhomogeneity with regard to the fiber type distribution in the VL of young males and an Inability to predict the fiber composition of a muscle with a single biopsy sample.The optical densities (OD) of the same 50 type I and 50 type II fibers were determined In each of three PAS stained serial sections per biopsy using a computer integrated photometric system. Mean total, fiber type specific, and Individual fiber OD did not differ significantly between the serial sections although a variability was observed. This variability appears to be primarily due to differences In sectional thickness. The comparison of biochemically determined glycogen content (41.0 - 191.0 mmol.kg-lwet weight) to mean total OD in sections from the same samples resulted in a poor relationship (r=0.47) between the two methods for the quantification of muscle glycogen. These results Indicate a variability in PAS stain intensity between serial sections of muscle biopsies and an inability to quantify muscle glycogen concentrations with the photometric determination of OD of the PAS stain in cross sections of muscle. / School of Physical Education
| Identifer | oai:union.ndltd.org:BSU/oai:cardinalscholar.bsu.edu:handle/183885 |
| Date | January 1990 |
| Creators | Benedict, Michael A. |
| Contributors | Ball State University. School of Physical Education., Costill, David L. |
| Source Sets | Ball State University |
| Detected Language | English |
| Format | vii, 93 leaves : ill. ; 28 cm. |
| Source | Virtual Press |
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