Se propuso detectar el gen de la adhesina p97 en cepas peruanas de M. hyopneumoniae obtenidas de lesiones macroscópicas sugerentes de Neumonía Enzoótica Porcina (NEP), en cerdos procedentes de granjas de crianza intensiva mediante la técnica de PCR y asociarlo con las lesiones microscópicas. Se colectaron en un camal de Lima 30 muestras de tejido pulmonar de cerdos de granjas de crianza intensiva procedentes de diferentes zonas geográficas de la costa del Perú. Las muestras se procesaron para análisis histopatológico y PCR. Se amplificó por PCR un fragmento, que incluía las regiones repetitivas R1 y R2, del gen p97 mediante primers diseñados para este estudio, brindados por la UNAM. Las lesiones microscópicas fueron clasificadas de 0-4 según escala de severidad propuesta por Piffer y Brito (1991) que las clasifica de acuerdo al grado de hiperplasia de agregados linfoides asociados a bronquios, bronquiolos y vasos sanguíneos (BALT), donde los grados de BALT de 3 y 4 son sugerentes de NEP. A la histopatología se observó que 18 muestras tuvieron grados de BALT 3 y 4, y a la PCR fueron positivas 14 de 30 muestras. El análisis estadístico reveló que la detección por PCR y las lesiones microscópicas no fue significativo.
Palabras clave: Porcinos, Mycoplasma hyopneumoniae, NEP, BALT, PCR, adhesina P97. / --- Set out to detect the gene of adhesin P97 in Peruvian strains of M. hyopneumoniae obtained from injuries macroscopic suggestive of swine enzootic pneumonia (NEP), pigs free-range intensive breeding by the PCR technique and associate it with the microscopic lesions. 30 Samples of lung tissue of pigs of intensive breeding farms from different geographical areas of the coast of the Perú collected in a slaughterhouse in Lima. The samples were processed for histopathological and PCR analysis. A fragment, which included repeating regions R1 and R2, the gene p97 using primers designed for this study, provided by the UNAM is amplified by PCR. Microscopic lesions were classified 0-4 according to scale of severity proposed by Piffer and Brito (1991), which classifies them according to the degree of hyperplasia of aggregated lymphoid associated with bronchi, Bronchioles, and blood vessels (BALT), where the degrees of BALT of 3 and 4 are suggestive of NPA. In the histopathology was observed that 18 samples had degrees of BALT 3 and 4, and PCR were positive 14 of 30 samples. Statistical analysis revealed that the detection by PCR and microscopic lesions was not significant.
Keywords: swine, Mycoplasma hyopneumoniae, NEP, BALT, PCR, adhesin P97.
| Identifer | oai:union.ndltd.org:Cybertesis/oai:cybertesis.unmsm.edu.pe:cybertesis/1573 |
| Date | January 2012 |
| Creators | Ramos García, Luis Miguel |
| Contributors | Maturrano, Lenin |
| Publisher | Universidad Nacional Mayor de San Marcos |
| Source Sets | Universidad Nacional Mayor de San Marcos - SISBIB PERU |
| Language | Spanish |
| Detected Language | English |
| Type | info:eu-repo/semantics/bacherlorThesis |
| Source | Universidad Nacional Mayor de San Marcos, Repositorio de Tesis - UNMSM |
| Rights | info:eu-repo/semantics/openAccess |
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