During vertebrate gastrulation, a well-orchestrated series of cell movements leads to the formation of the three germ layers: ectoderm, mesoderm and endoderm. In zebrafish, a model organism for vertebrate development, the mesendodermal progenitor cells separate from the ectodermal cells and migrate towards the animal pole. To identify proteins controlling these processes, I used a comparative proteomic approach following two alternative strategies: (1) Based on the notion that Wnt11 regulates cell movement and morphology during gastrulation independent of transcriptional regulation, I performed a screen aimed at the identification of proteins phosphorylated upon Wnt11 signalling. To regulate Wnt11 expression tightly, I engineered a transgenic slb/wnt11-/- fish line expressing wnt11 under the control of a heat shock promoter. Using this line, I performed a quantitative comparison of protein phosphorylation with or without Wnt11 pathway activation by analysing 32P-labelled embryo extracts on 2D gels. (2) Since these experiments did not reveal any Wnt11 targets, I addressed, in the second approach, proteomic differences causal for the changes in cell adhesion and motility observed in mesendodermal cells upon involution. Quantitative 2D gel analysis comparing ectodermal and mesendodermal cells revealed 37 significantly regulated spots, 36 of which I identified by mass spectrometry. Interestingly, the majority of these proteins were not regulated on a transcriptional level as determined by an accompanying microarray analysis confirming the complementary nature of proteomics and transcriptomics. Among the identified targets, several proteins, including Ezrin2, had previously been assigned a cytoskeleton-related function. I characterised Ezrin2 in more detail showing that Ezrin2 is specifically activated by phosphorylation in mesendodermal cells and that it is required for proper gastrulation movements. In the course of this study, I developed techniques for proteomic analysis of early zebrafish embryos, including a protocol to remove the yolk. I identified several cytoskeleton-related proteins in a comparative proteomic screen for regulators of gastrulation movements. The subsequent characterisation of Ezrin2 confirmed the power of proteomics for the analysis of developmental processes. In conclusion, this work provides a foundation to study developmental and cell biological questions in early zebrafish embryos using proteomics.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:24691 |
| Date | 19 April 2006 |
| Creators | Link, Vinzenz |
| Contributors | Heisenberg, Carl-Philipp, Hoflack, Bernard, Spaink, Herman |
| Publisher | Technische Universität Dresden |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | doc-type:doctoralThesis, info:eu-repo/semantics/doctoralThesis, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
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