In order to maintain a multicellular organism cells need to interact and communicate with each other. Signalling cascades such as the Bone Morphogenic Protein (BMP) and Hedgehog (Hh) signalling pathways therefore play essential roles in development and disease. Intercellular signalling also underlies the function of stem cell niches, signalling microenvironments that regulate behaviour of associated stem cells. Range and intensity of the niche signal controls stem cell proliferation and differentation and must therefore be strictly regulated. The testis and ovary of the fruit fly Drosophila melanogaster are established models of stem cell niche biology.
In the apical tip of the testis, germ line stem cell (GSCs) and somatic cyst stem cells (CySCs) are arranged around a group of postmitotic somatic cells termed hub. While it is clear which signals regulate GSC maintenance it is unclear how these signals are spatially regulated. Here I show that BMP signalling is specifically activated at the interface of niche and stem cells. This local activation is possible because the transport of signalling and adhesion molecules is coupled and directed towards contact sites between niche and stem cells. I further show that the generation of the BMP signal in the wing disc follows the same mechanism.
Hh signalling controls somatic stem cell populations in the Drosophila ovary and the mammalian testis. However, it was unknown what role Hh might play in the fly testis, where the components of this signalling cascade are also expressed. Here I show that overactivation of Hh signalling leads to an increased proliferation and an expansion of the cyst stem cell compartment.
Finally, while the major components of the Hh signalling pathway are known, detailed knowledge of how signal transduction is implemented at the cell biological level is still lacking. Here, I show that localisation of the key signal transducer Smo to the plasma membrane is sufficient for phosphorylation of its cytoplasmic tail and downstream pathway activation. Using advanced, microscopy based biophysical methods I further demonstrate that Smo clustering is, in contrast to the textbook model, independent of phosphorylation.:Summary 1
List of publications 3
1 Introduction 9
Aims of the thesis 15
2 Generation of a local BMP signal in testis and wing disc 17
2.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
2.1.1 Stem cells and stem cell niches . . . . . . . . . . . . . . 19
2.1.2 The Drosophila testis stem cell niche . . . . . . . . . . 20
2.1.3 BMP signalling in the fly . . . . . . . . . . . . . . . . . 23
2.2 Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
2.2.1 The BMP niche signal is transduced locally at adherens junctions 25
2.2.2 Generation of the local BMP niche signal . . . . . . . . 30
2.2.3 Exocyst involvement in long-range BMP signalling . . 34
2.3 Discussion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
3 Hedgehog pathway overactivation in the testicular niche 41
3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
3.1.1 The role of Hedgehog in the fly . . . . . . . . . . . . . 43
3.2 Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
3.2.1 Overexpression of Hh increases the CySC number and expands their range 45
3.3 Discussion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
4 Visualization of Smo phosphorylation and biophysical detection of Smo clustering 49
4.1 Introduction (part I) . . . . . . . . . . . . . . . . . . . . . . . 51
4.1.1 Hedgehog signalling in the fly . . . . . . . . . . . . . . 51
4.1.2 Reception and transduction of the Hh signal by Ptc and Smo 54
4.2 Results (part I) . . . . . . . . . . . . . . . . . . . . . . . . . . 56
4.2.1 A fluorescent reporter for Drosophila Smo tail phosphorylation 56
4.2.2 Smo phosphorylation and localisation in the salivary gland 61
4.2.3 Smo localisation in cultured insect cells . . . . . . . . . 63
4.2.4 Smo membrane localisation and phosphorylation . . . . 65
4.3 Introduction (part II) . . . . . . . . . . . . . . . . . . . . . . . 67
4.3.1 Fluorescence correlation spectroscopy (FCS) . . . . . . 67
4.3.2 Dual-color fluorescence cross-correlation spectroscopy (FCCS) 72
4.3.3 Artefacts in FCS/FCCS . . . . . . . . . . . . . . . . . 73
4.4 Results (part II) . . . . . . . . . . . . . . . . . . . . . . . . . . 79
4.4.1 Smo clustering measured by FCCS . . . . . . . . . . . 79
4.5 Discussion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:28903 |
| Date | 01 July 2015 |
| Creators | Raabe, Isabel |
| Contributors | Bökel, Christian, Rödel, Gerhard, Brand, Michael, Reichardt, Werner, Technische Universität Dresden |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | doc-type:doctoralThesis, info:eu-repo/semantics/doctoralThesis, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
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