Multicellular organisms need to control their size throughout development and adult life in the face of challenges such as rapid growth. Unraveling the mechanisms that regulate tissue growth in epithelial tissues, in order to generate organs of correct size and proportion, remains a crucial goal of developmental biology. A suitable epithelial tissue for studying tissue growth in Drosophila, is the proliferative monolayer epithelial sheet of imaginal wing discs, which gives rise to the adult wing. The Hippo signaling pathway regulates tissue growth in wing development. There are several observations that link tissue growth/Hippo signaling with cell polarity and the actin cytoskeletal organization.
The aim of this thesis was the study of the interplay between cell polarity, cytoskeletal organization and tissue growth. To gain further insight into how apical polarity proteins regulate tissue growth, an enhancer/suppressor screen that was previously conducted in our lab by Linda Nemetschke, was used. The screen was based on the modification of a dominant smaller wing phenotype induced upon overexpression of CrbextraTM-GFP. One of the enhancers identified in this screen is a gene called big bang (bbg). The absence of bbg results in smaller wings with a slower cell cycle and increased apoptosis in wing discs. bbg encodes a protein expressed in the apical cortex in wing disc cells and is required for the proper localization of apical proteins, like Crb, in wing disc epithelia. Bbg is also in the same complex with Spaghetti Squash (Sqh) in the apical cortex of the wing disc epithelia. sqh encodes an actin-binding protein that has actin cross-linking and contractile properties. Bbg stabilizes Sqh in the apical compartment of the cell. It is reported that both Crb and Sqh regulate tissue growth through the Hippo signaling pathway. In conclusion, Bbg regulates wing tissue growth, acting as a scaffolding molecule, through the proper localization of apical components of the cells like Crb and the cytoskeletal component Sqh.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:29434 |
| Date | 01 April 2016 |
| Creators | Tsoumpekos, Georgios |
| Contributors | Knust, Elisabeth, Dahmann, Christian, Technische Universität Dresden |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | doc-type:doctoralThesis, info:eu-repo/semantics/doctoralThesis, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
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