The effect of six heavy metals, namely, silver (Ag), lead (Pb), palladium (Pd), copper (Cu),
nickel (Ni), and chromium (Cr), on phosphorus (P) uptake by yeast was investigated by
single-cell analysis using inductively coupled plasma time-of-flight mass spectrometry
(SC-ICP-TOF-MS). It was found that the P content in cells with 1.55 g L1 P feeding
after P starvation was increased by 70% compared to control cells. Heavy metals at
10 ppm, except Cu, had a negative impact on P accumulation by cells. Pd reduced the P
content by 26% in single cells compared to control cells. Metal uptake was strongest for
Ag and Pd (0.7 1012 L cell1) and weakest for Cr (0.05 1012 L cell1). Exposure
to Cr markedly reduced (50%) Mg in cells and had the greatest impact on the intrinsic
element composition. The SC-ICP-TOF-MS shows the diversity of elemental content
in single cells: for example, the P content under standard conditions varied between
12.4 and 890 fg cell1. This technique allows studying both the uptake of elements and
sublethal effects on physiology at a single-cell level.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:85837 |
| Date | 07 June 2023 |
| Creators | Quin, Wen, Stärk, Hans-Joachim, Müller, Susann, Reemtsma, Thorsten |
| Publisher | Frontiers Media S.A. |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 870931 |
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