We investigated the fungus Aspergillus fumigatus PD-18 responses when subjected to
the multimetal combination (Total Cr, Cd2C, Cu2C, Ni2C, Pb2C, and Zn2C) in synthetic
composite media. To understand how multimetal stress impacts fungal cells at the
molecular level, the cellular response of A. fumigatus PD-18 to 30 mg/L multimetal stress
(5 mg/L of each heavy metal) was determined by proteomics. The comparative fungal
proteomics displayed the remarkable inherent intracellular and extracellular mechanism
of metal resistance and tolerance potential of A. fumigatus PD-18. This study reported
2,238 proteins of which 434 proteins were exclusively expressed in multimetal extracts.
The most predominant functional class expressed was for cellular processing and
signaling. The type of proteins and the number of proteins that were upregulated due
to various stress tolerance mechanisms were post-translational modification, protein
turnover, and chaperones (42); translation, ribosomal structure, and biogenesis (60); and
intracellular trafficking, secretion, and vesicular transport (18). In addition, free radical
scavenging antioxidant proteins, such as superoxide dismutase, were upregulated upto
3.45-fold and transporter systems, such as protein transport (SEC31), upto 3.31-fold
to combat the oxidative stress caused by the multiple metals. Also, protein–protein
interaction network analysis revealed that cytochrome c oxidase and 60S ribosomal
protein played key roles to detoxify the multimetal. To the best of our knowledge, this
study of A. fumigatus PD-18 provides valuable insights toward the growing research in
comprehending the metal microbe interactions in the presence of multimetal. This will
facilitate in development of novel molecular markers for contaminant bioremediation.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:86431 |
| Date | 11 July 2023 |
| Creators | Dey, Priyadarshini, Malik, Anushree, Singh, Dileep Kumar, Haange, Sven-Bastiaan, von Bergen, Martin, Jehmlich, Nico |
| Publisher | Frontiers Media |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 1664-302X, 872576 |
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