Background: Human head and neck squamous cell carcinoma (HNSCC) fundamentally vary in their susceptibility to different cytotoxic drugs and treatment modalities. There is at present no clinically accepted test system to predict the most effective therapy for an individual patient. Methods: Therefore, we established tumor-derived slice cultures which can be kept in vitro for at least six days. Upon treatment with cisplatin, docetaxel and cetuximab, slices were fixed and paraffin sections were cut for histopathological analysis.
Results: Apoptotic fragmentation, activation of caspase 3, and cell loss were observed in treated tumor slices. Counts of nuclei per field in untreated compared to treated slices deriving from the same tumor allowed estimation of the anti-neoplastic activity of individual drugs on an individual tumor.
Conclusion: HNSCC-derived slice cultures survive well in vitro and may serve to improve personalized therapies, but also to detect mechanisms of tumor resistance by harvesting surviving tumor cells after treatment.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa.de:bsz:15-qucosa-160372 |
| Date | 05 February 2015 |
| Creators | Gerlach, Magdalena |
| Contributors | Institut für Anatomie, Medizinische Fakultät, Prof. Dr. med. Ingo Bechmann, Prof. Dr. med. Torsten Remmerbach, Prof. Dr. med. Michael Fuchs |
| Publisher | Universitätsbibliothek Leipzig |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | deu |
| Detected Language | English |
| Type | doc-type:doctoralThesis |
| Format | application/pdf |
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