The clinical consequences of ionizing radiation exposure remain one of the leading causes of death in the United States. Much research has been carried out to discover a potential countermeasure for acute radiation syndrome (ARS) without success. The United States Food and Drug Administration (US FDA) has not accepted any effective and harmless ionizing radiation therapy agents (radioprotectors) for treating ARS. It has recently been discovered that g-tocotrienol (GT-3), one of the E vitamers chiefly present in palm oil, has radioprotective abilities in mice and nonhuman primate (NHP) models. Though GT-3 is one of the most promising countermeasures discovered, the separation and purification from other vitamers or its matrix is difficult. This has limited its characterization, derivatization, and biomedical application. We have therefore designed novel chromatographic methods to optimize separation and purification. Thin layer chromatography (TLC) was used to ascertain the best solvent system for column chromatography (CC). Exactly 8% ethyl acetate in hexane employed in TLC and CC resulted in good separation (Rf ≥ 0.3) and purification. Various fractions presumed to contain GT-3 were collected and analyzed to confirm the exact structure using 1H NMR, 13C NMR, DEPT, and GC-MS. Results obtained so far have revealed the exact structure of the compound. However, some traces of impurities have been indicated by the NMR outcomes; therefore, high-performance liquid chromatography (HPLC) will be used to maximize GT-3 purification. This present study will be instrumental in elucidating the biochemical structure of various complex plant bioactive components that are hard to isolate and analyze. It is envisioned that this work will help to erase the knowledge deficit in medicinal chemistry and assist in the development of new medications for ARS.
| Identifer | oai:union.ndltd.org:ETSU/oai:dc.etsu.edu:asrf-1286 |
| Date | 12 April 2019 |
| Creators | Fobi, Kwabena, Lynn, Bronson, Shilabin, Abbas Gholipour |
| Publisher | Digital Commons @ East Tennessee State University |
| Source Sets | East Tennessee State University |
| Detected Language | English |
| Type | text |
| Source | Appalachian Student Research Forum |
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