Adult male albino Sprague Dawley rats were fed diets containing 15 % of casein with a constant 0.62 % of supplemental methionine, and three levels of inorganic sulfate, 0.0002 %, 0.02 %, and 0.42 %. The polyamines (putrescine, spermidine, and spermine) and the controlling enzymes for their biosynthesis, ornithine decarboxylase (ODC) and S-adenosyl-methionine decarboxylase (SAMD) were evaluated in liver tissue homogenates following a 17 day dietary period. There was no increase in the activity of ODC or the tissue concentration of putrescine in the liver tissue of rats fed the diet low in sulfate. There was an increase in SAMD activity and the concentration of spermidine and spermine. The activity of both ODC and SAMD and the tissue concentration of putrescine, spermidine, and spermine increased in the liver tissue from rats fed the diet high in inorganic sulfate. These data coupled with previous observations suggest that the metabolic effects of diets low in inorganic sulfate are probably not mediated through the regulatory activity of the polyamines. However, these data allow the suggestion that the metabolic alterations observed in rats fed diets high in inorganic sulfate may be due to the regulatory action of the polyamines.
| Identifer | oai:union.ndltd.org:ETSU/oai:dc.etsu.edu:etsu-works-14662 |
| Date | 01 January 1985 |
| Creators | Acuff, Robert V., Smith, J. T. |
| Publisher | Digital Commons @ East Tennessee State University |
| Source Sets | East Tennessee State University |
| Detected Language | English |
| Type | text |
| Source | ETSU Faculty Works |
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