Stem cell-based therapies are promising in regenerative medicine. However, the
success of cell therapy is greatly limited by the low engraftment rate to the target
tissues.
The present study demonstrated that human mesenchymal stem cells (hMSCs)
were subjected to a self selection process via microencapsulation in collagen
barrier when they were induced to migrate out from this barrier. While retaining
the immuophenotype and self renewal capacity, the selected hMSCs showed a
significantly better in vitro migratory response of than those cultured in
traditional monolayer. The migratory response could be controlled by varying the
fabrication parameters of the collagen barrier, including initial collagen
concentration and cells seeding density. Affinity to adhere on endothelial cells
layer is another engraftment related property. Significant difference was observed
between these selected hMSCs and hMSCs in monolayer culture.
In order to investigate the engraftment potential of the selected hMSCs, an
animal model was performed. The selected hMSCs were transplanted
intravenously into NOD/SCID mice under partial hepatectomy. Presence of
human cells in the residual liver was determined by the presence of human
HLA-ABC using flow cytometry after 48 hours, 1 week and 1 month.
Engraftment of the selected hMSCs was significantly higher than that of
monolayer cultured hMSCs in time point of 1 month. It demonstrated that the
selected hMSCs favor the engraftment to the injured liver. Further investigation
is required to determine the fate of the engrafted hMSCs in order to truly confirm
their therapeutic potential.
The current work demonstrated that collagen-hMSCs microsphere could act as a
barrier to select hMSCs with enhanced in vitro migratory response and in vivo
engraftment properties. These findings may contribute towards the development
of better stem cell therapies. / published_or_final_version / Mechanical Engineering / Master / Master of Philosophy
| Identifer | oai:union.ndltd.org:HKU/oai:hub.hku.hk:10722/174488 |
| Date | January 2012 |
| Creators | Wong, Mei-yi., 王美兒. |
| Contributors | Chan, BP |
| Publisher | The University of Hong Kong (Pokfulam, Hong Kong) |
| Source Sets | Hong Kong University Theses |
| Language | English |
| Detected Language | English |
| Type | PG_Thesis |
| Source | http://hub.hku.hk/bib/B47753080 |
| Rights | The author retains all proprietary rights, (such as patent rights) and the right to use in future works., Creative Commons: Attribution 3.0 Hong Kong License |
| Relation | HKU Theses Online (HKUTO) |
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