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Previous issue date: 2017-03-20 / Myelofibrosis is a hematological disease inserted in the group of myeloproliferative neoplasias. It has as main characteristic fibrosis of the bone marrow, consequence of a variety of histological changes presented in the medullary microenvironment. The pathophysiology of myelofibrosis involves the activation of signal transduction pathways and nowadays several mutations such as JAK2V617F, CalR and MPL have been associated with this process. Recent studies have shown that microvesicles produced by body cells may be associated with the cellular communication process. These microvesicles carry in their content proteins, lipids and RNA capable of regulating diverse cellular processes. The literature shows that several miRNAs present in the microvesucular content can regulate the hematopoiesis of normal stem cells and also of compromised progenitors, having an important role in the pathogenesis of some acquired hematological neoplasias such as myeloproliferative diseases. With the objective of investigating the presence of specific miRNAs in microvesicles excreted in the peripheral blood plasma of patients with myelofibrosis, microvesicles were isolated from the plasma by ultracentrifugation and by means of molecular biology techniques it was possible to validate the presence of these microbes. Real-time PCR assays were performed to evaluate the expression of specific miRNAs (miR-146b, miR-221, miR-143 and miR-150) in the microvesic contents. In order to analyze the functional activity of MVs, the cell migration assay was performed, aiming at the progression of pathology and tumor invasiveness. The results show that there is no increase in the microvesicular concentration in peripheral blood when comparing the group of patients with the control group. The data found show that miR-146b and miR-150 are differentially expressed in patients with myelofibrosis, being little expressed. However, miR-221 and miR-143 didn???t show amplification in the technique, which leads to the conclusion that these miRNAs are specific to the medullary microenvironment or aren???t released in the microvesicular content. Regarding the migration test, the result shows that there was no influence of the microvesicles in the process of cell proliferation and migration and it can be considered that the microvesicles isolated in our study may not present this role because they are not exclusively of the bone marrow environment. As future prospects, assays with microvesicles removed from the bone marrow can be performed. / A Mielofibrose ?? uma doen??a hematol??gica inserida no grupo de neoplasias mieloproliferativas. Possui como principal caracter??stica a fibrose da medula ??ssea, consequ??ncia de uma variedade de mudan??as histol??gicas apresentadas no microambiente medular. A fisiopatog??nese da Mielofibrose envolve a ativa????o de vias de transdu????o de sinais e nos ??ltimos anos v??rias muta????es como a JAK2V617F, a CalR e a MPL foram associadas a doen??a. Estudos recentes demonstraram que microves??culas produzidas por c??lulas do organismo podem estar associadas ao processo de comunica????o celular. Estas microves??culas transportam em seu conte??do prote??nas, lip??dios e RNA capazes de regular variados processos celulares. A literatura mostra que diversos miRNAs presentes no conte??do microvesucular podem regular a hematopoiese de c??lulas-tronco normais e tamb??m de progenitores comprometidos, tendo um papel importante na patog??nese de algumas neoplasias hematol??gicas adquiridas como as doen??as mieloproliferativas. Com objetivo de investigar a presen??a de miRNAs espec??ficos em microves??culas excretadas no plasma de sangue perif??rico de pacientes portadores de mielofibrose, microves??culas foram isoladas do plasma por m??todo de ultracentrifuga????o e por meio de t??cnicas de biologia molecular foi poss??vel confirmar a presen??a destas. Ensaios com PCR em tempo real foram realizados afim de avaliar a express??o de miRNAs espec??ficos (miR-146b, miR-221, miR-143 e miR-150) no conte??do das microves??culas e para analisar a atividade funcional das M.Vs foi realizado o ensaio de migra????o celular que visa avaliar progress??o da patologia e a invasividade tumoral. Os resultados obtidos mostram que n??o h?? aumento da concentra????o microvesicular em sangue perif??rico quando comparado o grupo de pacientes com o grupo controle. Os dados encontrados mostram que o miR-146b e o miR-150 apresentam-se diferencialmente expressos em pacientes com mielofibrose, com pouca express??o. J?? o miR-221 e o miR-143 n??o apresentaram detec????o na t??cnica, o que o que leva a concluir que estes miRNAs s??o pr??prios do microambiente medular ou n??o s??o liberados no conte??do microvesicular. Quanto a ensaio de migra????o o resultado mostra que n??o houve influ??ncia das microves??culas no processo de prolifera????o e migra????o celular e pode ser considerado que as microves??culas isoladas em nosso estudo podem n??o apresentar este papel por n??o serem exclusivamente do ambiente medular. Como perspectivas futuras, ensaios com microves??culas retiradas da medula ??ssea poder??o ser realizados.
| Identifer | oai:union.ndltd.org:IBICT/oai:bdtd.ucb.br:tede/2286 |
| Date | 20 March 2017 |
| Creators | Rodrigues, Leane Perim |
| Contributors | Pereira, Rinaldo Wellerson, Mascarenhas, Cintia do Couto |
| Publisher | Universidade Cat??lica de Bras??lia, Programa Strictu Sensu em Ci??ncias Gen??micas e Biotecnologia, UCB, Brasil, Escola de Sa??de e Medicina |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UCB, instname:Universidade Católica de Brasília, instacron:UCB |
| Rights | info:eu-repo/semantics/openAccess |
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