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Previous issue date: 2001-08-31 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / A lagarta elasmo, Elasmopalpus lignosellus (Lepidoptera: Pyralidae), e a lagarta do cartucho, Spodoptera frugiperda (Lepidoptera: Noctuidae), são pragas relevantes, particularmente da cultura de milho. O presente estudo objetivou estudar regiões do DNA de E. lignosellus e avaliar milhos transgênicos, expressando as toxinas Cry 1 A(b), Cry 9C e Cry 1F, como potencial de controle desta lagarta, além de estudos de tolerância às toxinas de Cry 1 A(b) em S. frugiperda avaliando a herdabilidade desta tolerância e diferenciação, por marcadores AFLP, das populações de S. frugiperda que apresentaram tolerância e susceptibilidade à toxina avaliada. É possível diferenciar as populações brasileira e americana de E. lignosellus com PCR-RFLP e sequenciamento do gene mitocondrial COI. Milhos Bt, expressando as toxinas Cry 1 A(b), Cry 9C e Cry 1F, estão protegidos contra o ataque da lagarta elasmo. S. frugiperda mostrou aumento na tolerância à toxina Cry 1A(b) após seleção por quatro gerações e registrou-se um componente hereditário nesse fenômeno. Os dados de AFLP mostram haver diferença entre as populações tolerantes e susceptíveis. / The lesser cornstalk borer (LCB), Elasmopalpus lignosellus (Lepidoptera: Pyralidae) and the fall armyworm (FAW), Spodoptera frugiperda (Lepidoptera: Noctuidae) are important insect pest. To contribute to managing programs of these insects we propose to study mtDNA region in LCB allowing differentiation on populations from Brazil and USA, and evaluate the efficiency of Bt corn to control this pest. Also, to determine the FAW tolerance to Cry1A(b) toxin and to study the genetic polymorphism of survival of fall armyworm using AFLP technique. The results show that is possible to distinguish LCB populations from Brazil and USA using COI mitochondrial gene by PCR-RFLP and sequencing, and that Bt transgenic maize were able to protect the plants against LCSB damage. Increased tolerance to Cry 1A(b) protein was found in populations of fall armyworm after selection for four generations with Cry 1A(b)toxin. Tolerance of FAW to Cry1 A(b) had a heritage component in the studied generations. The following work, also, describes efforts to detect genetic polymorphisms between survivors.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:123456789/11352 |
| Date | 31 August 2001 |
| Creators | Vilella, Francys Mara Ferreira |
| Contributors | Waquil, José Magid, Santos, Jorge Abdala Dergam dos, Vilela, Evaldo Ferreira |
| Publisher | Universidade Federal de Viçosa |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | Portuguese |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Source | reponame:Repositório Institucional da UFV, instname:Universidade Federal de Viçosa, instacron:UFV |
| Rights | info:eu-repo/semantics/openAccess |
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