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Previous issue date: 2014-07-29 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / ARAUJO, Karla Emanuelle Campos. Isotopic fractionation of 15N in nitrogen fixation in soybean due to light intensity and Bradyrhizobium spp. strain inoculated. 2014. 40f. Dissertation (Master in Plant Science). Institute of Agronomy, Department of Plant Science, Federal Rural University of Rio de Janeiro, Serop?dica, RJ, 2014.
This work aimed to study the isotopic fractionation of N from BNF in stems function and light intensity in soybean plant symbiosis with Bradyrhizobium. The 'B' value was evaluated for ten Bradyrhizobium strains, as well as their interaction with the soybean plant (Glycine max L.). The experiment was conducted at Embrapa greenhouse Agrobiologia, Serop?dica, RJ. The soybean plants inoculated with the different strains of Bradyrhizobium and a control not inoculated, were grown under conditions similar to Leonard vessels using substrate of sand and vermiculite in a 2: 1 (v / v). In the second experiment to assess the value 'B' of the resulting symbiosis BNF among cultivars (cv) of soybean BRS 133, BRS 184, MONSOY9144 nine Bradyrhizobium. Soybean plants in all the treatments, were grown under the same conditions, the soybean cultivation was performed in vessels Leonard with sand and perlite substrate in the proportion 1: 1 (v / v). In both experiments after 47 days of planting the plants were harvested and calculated the value 'B'. In the third experiment evaluated the effect of different strains of Bradyrhizobium and the reduction in the intensity of light on natural abundance of 15N N2 fixed by the strains in symbiosis with soybean cv BRS 133. An experiment was conducted in the experimental field of Embrapa Agrobiology. The soybean plants inoculated with 11 Bradyrhizobium and treatment uninoculated were grown under similar conditions in plastic pots with sand substrate and pearlite in the proportion 1: 1 (v / v) .After 75 days after planting the plants were collected and calculated the value 'B'.No experiment 1, the values of ?Bpa? ranged between -2 and -4 ?, according to the strain used. In experiment 2, the values of ?Bpa? showed no difference between the soybean cultivars inoculated with Bradyrhizobium as the isotopic abundance of 15N in plants grown entirely dependent on FBN. There was a tendency for the abundance of 15N values in ?Bpa? treatment of plants inoculated with strains of B. elkanii to be less negative than in the case of plants inoculated with B. japonicum. In experiment 3 the values of ?B?PA showed natural 15N abundance values significantly more negative than the shaded plants. There was a tendency for the abundance of 15N in the shoot (and values of ?Bpa?) treatment of the plants inoculated with B. japonicum strains to be less negative than in the case of plants inoculated with B. elkanii. For the determination of the 'B' value, the plants should be in the same stage of maturity that being used to sample the plants in the field, to assess the value 'B' in low light intensity conditions (green house) can lead to the result of a 'B' value less negative than that given in full sun and so probably less appropriate uses it to calculate the contribution of BNF in legume under field conditions. / ARAUJO, Karla Emanuelle Campos. Fracionamento isot?pico do 15N na fixa??o biol?gica de nitrog?nio na soja em fun??o da intensidade de luz e estirpe de Bradyrhizobium spp. inoculada. 2014. 40f. Disserta??o (Mestrado em Fitotecnia). Instituto de Agronomia, Departamento de Fitotecnia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ, 2014.
Este trabalho teve como objetivo estudar o fracionamento isot?pico do N proveniente da FBN, em fun??o de estipes e intensidade de luz na simbiose da planta de soja com Bradyrhizobium. O valor ?B? foi avaliado para dez estirpes de Bradyrhizobium, assim como sua intera??o com a planta de soja (Glycine max, L.). Foi conduzido o experimento na casa de vegeta??o da Embrapa Agrobiologia, Serop?dica, RJ. As plantas de soja inoculadas com as diferentes estirpes de Bradyrhizobium e um controle, n?o inoculado, foram crescidas em condi??es semelhantes em vasos Leonard, utilizando substrato de areia e vermiculita na propor??o 2:1 (v/v). No segundo experimento para avaliar o valor ?B? da FBN resultante da simbiose entre as cultivares (cv) de soja BRS 133, BRS 184, MONSOY9144 e nove estirpes de Bradyrhizobium. As plantas de soja para todos os tratamentos, foram crescidas em condi??es iguais, o cultivo da soja foi realizado em vasos Leonard, utilizando substrato de areia e perlita na propor??o 1:1 (v/v). Nos dois experimentos ap?s 47 dias do plantio as plantas foram colhidas e calculou o valor ?B?. No terceiro experimento avaliou-se o efeito de diferentes estirpes de Bradyrhizobium e da redu??o na intensidade de luz sobre abund?ncia natural de 15N do N2 fixado pelas estirpes em simbiose com a cv. de soja BRS 133. Foi conduzido um experimento no campo experimental da Embrapa Agrobiologia. As plantas de soja inoculadas com 11 estirpes de Bradyrhizobium e um tratamento n?o inoculado, foram crescidas em condi??es semelhantes em vasos pl?sticos, utilizando substrato de areia e perlita na propor??o 1:1 (v/v). Ap?s 75 dias do plantio as plantas foram colhidas e calculou-se o valor ?B?. No experimento 1, os valores de ?Bpa? variaram entre -2 e -4 ?, de acordo com a estirpe usada. No experimento 2, os valores de ?Bpa? n?o apresentaram diferen?a entre as cultivares de soja inoculadas com os Bradyrhizobium quanto a abund?ncia isot?pica de 15N nas plantas crescida inteiramente dependente da FBN. Houve uma tend?ncia para a abund?ncia de 15N nos valores de ?Bpa? das plantas dos tratamentos inoculados com estirpes de B. elkanii a serem menos negativo do que no caso de plantas inoculadas com B. japonicum. No experimento 3 os valores de ?Bpa? apresentaram valores de abund?ncia natural de 15N significativamente mais negativos do que as plantas sombreadas. Houve uma tend?ncia para a abund?ncia de 15N na parte a?rea (e os valores de ?Bpa?) das plantas dos tratamentos inoculados com estirpes de B. japonicum a serem menos negativo do que no caso de plantas inoculadas com B. elkanii. Para ? determina??o do valor ?B?, as plantas devem estar no mesmo est?gio de maturidade que as que est?o sendo utilizadas para amostrar as plantas no campo, a avalia??o do valor ?B? em condi??es de intensidade de luz reduzida (casa de vegeta??o) pode levar ao resultado de um valor ?B? menos negativo do que aquele determinado em pleno sol e assim provavelmente menos apropriado utiliza-lo, para calcular a contribui??o da FBN em leguminosa em condi??es de campo.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:jspui/1999 |
| Date | 29 July 2014 |
| Creators | Ara?jo, Karla Emanuelle Campos |
| Contributors | Urquiaga Caballero, Segundo Sacramento, Boddey, Robert Michael, Jacob Neto, Jorge, Soares, Lu?s Henrique de Barros |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Programa de P?s-Gradua??o em Fitotecnia, UFRRJ, Brasil, Instituto de Agronomia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | ALBAREDA, M.; RODR?GUES ? NAVARO, D. N.;TEMPRANO, F. J. Soybean inoculation: Dose N fertilizer supplementation and rhizobia persistence in soil. Field crops research, 113: 352-356, 2009. ALVES, B. J. R.; BODDEY, R. M.; URQUIAGA, S. The success of soybean in Brazil. Plant and Soil, 252:1-9, 2003. ALVES, B. J. R.; SANTOS, J. C. F.; BODDEY, R. M. M?todos de determina??o do nitrog?nio em solo e planta. In: HUNGRIA, M.; ARA?JO, R. S. Manual de m?todos empregados em estudos de microbiologia agr?cola. Bras?lia, DF: EMBRAPA/SPI, p. 449-470, 1994. (EMBRAPA-CNPAF. Documentos, 46). ALVES, B.J.R., Aplica??o da t?cnica de an?lise de solutos nitrogenados da seiva para a quantifica??o da fixa??o biol?gica de nitrog?nio em Desmodium ovalifolium CV. Itabela. Serop?dica, RJ. 1996. 150p. Tese (Doutorado em Agronomia - Ci?ncia do Solo)- Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Serop?dica-RJ. ALVES, B.J.R.; ZOTARELLI, L.; FERNANDES F. M.; HECKLER, J.C.; MACEDO, R.A.T.; BODDEY,R. M.; JANTALIA, C. P.; URQUIAGA, S. Fixa??o biol?gica de nitrog?nio e fertilizantes nitrogenados no balan?o de nitrog?nio em soja, milho e algod?o. Pesquisa agropecu?ria brasileira, Bras?lia, v.41, n.3, p.449-456, mar. 2006. ARA?JO, A. S. F.; CARVALHO, E. M. S. Fixa??o Biol?gica de Nitrog?nio. Centro de ci?ncias agr?rias, comunicado t?cnico, n. 11, p. 1-4, abril de 2006. ARNOLD, S.L., SCHEPERS, J.S. A simple roller-mill grinding procedure for plant and soil samples. Communications in Soil Science and Plant Analysis 35, 537e 545,2004. BETHLENFALVAY, G. S. & PHILLIPS, D. A. Variation in nitrogenase and hydrogenase activity of Alaska pea root nodules. Plant Physiology, 63: 816-820, 1979. BIZARRO, M. J. Simbiose e variabilidade de estirpes de Bradyrhizobium associadas a cultura da soja em diferentes manejos de solo. 2008. 107 f. Tese (Doutorado em Ci?ncia do Solo) - Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul, Porto Alegre. BODDEY R.M. Methods for quantification of nitrogen fixation associated with gramineae. CRC Crit. Rev. Plant Sci., 6: 209-266, 1987. BODDEY, R. M.; ALVES, B. J. R.; URQUIAGA, S. Quantifica??o da fixa??o biol?gica de nitrog?nio associada a plantas utilizando o is?topo 15N. In: HUNGRIA, M.; ARA?JO, R. S. (Ed.). Manual de m?todos em estudo de microbiologia agr?cola. Bras?lia, DF: EMBRAPA/SPI, 1994. p. 471-494. BODDEY, R.M. & VICTORIA, R.L. Estimation of biological nitrogen fixation associated with Brachiaria and Paspalum grasses using 15N-labelled organic matter and fertilizer. Plant and Soil, Dordretch, 90: 265-292. 1986. BODDEY, R.M.; PEOPLES, M.B.; PALMER, B.; DART, P .J. Use of the 15N natural abundance technique to quantify biological nitrogen fixation by woody perennials. Nutrient Cycling in Agroecosystems, v.57, p.235-270, 2000. 36 BREMNER, J.M. Total Nitrogen In: Black, C.A. et al. Ed. Methods of Soil Analysis, Parte 2, Chemical and Microbial Properties, American Society of Agronomy, 1144-1178, 1965. BULEN, W. A.; BURNS, R. C.; LeCOMTE, J. R. The nitrogenase system from Azotobacter : two enzime requirement for N2 reduction, ATP-dependente H2 evolution and ATP hidrolysis. Proceedings of the National Aacdemy of Sciences of the United States of America, 56: 979-986, 1966. BURRIS, R.H. The acetylene reduction technique. In: STEWART, D.P. Nitrogen fixation by free-living microorganisms, Cambridge: Cambridge University Press, 1975. p.249-258 (International Biological Program, 6). CASTOLDI, R.; CHARLO,CH. C. O.; VARGAS, P. F.; BRAZ, L. T.; CARR?O-PANIZZI, M. C.; MENDON?A, J. L. Influ?ncia do espa?amento entre plantas em caracter?sticas agron?micas de dois gen?tipos de soja-hortali?a de ciclo tardio em Jaboticabal-SP. Cient?fica, 37: 61-66, 2009. COMPANHIA NACIONAL DE ABASTECIMENTO-CONAB http://www.conab.gov.br/OlalaCMS/uploads/arquivos/12_12_06_09_10_01_boletim_portugues_dezembro_2014.pdf DILWORTH, M. J. Acetylene reduction by nitrogen-fixing preparations from Clostridium pasteurianum. Biochimica et Biophysica Acta, 127: 285-294, 1966. D?BEREINER, J.; FRANCO, A. A.; GUZMAN, I. Estirpes de Rhizobium japonicum de excepcional efici?ncia. Pesquisa Agropecu?ria Brasileira, 5: 55-161, 1970. DREVON, J. J., KALIA, V. C., HECKMANN, M. O. & SALSAC, L. Influence of the Bradyrhizobium japonicum hydrogenase on the growth of Glycine and Vigna species. Applied and Environmental Microbiology, 53: 610-612, 1987. EMPRESA BRASILEIRA DE PESQUISA AGROPECU?RIA - EMBRAPA. Tecnologias de produ??o da soja ? Regi?o central do Brasil 2001. Londrina, Embrapa Soja : Embrapa Cerrado : Embrapa Agropecu?ria Oeste : ESALQ, 2002. 199p. EVANS, H. J., HANUS, F. J., HAUGLAND, R. A., CANTRELL, M. A., XU, L. S., RUSSEL, F. J., LAMBERT, G. R. & HARKER, A. R. Hydrogen recycling in nodules affects nitrogen fixation and growth of soybeans. In: SHIBLES, R.ed. World Soybean Research Conference III Proceedings, Boulder, Westview Press, 1985. p. 935-942. EVANS, H. J., HARKER, A.R., PAPEN, H., RUSSELL, S.A., HANUS, F.J. & ZUBER, M. 1987 Physiology, biochemistry and genetics of the uptake hydrogenase in rhizobia. Annual Review of Microbiology, Palo Alto, v. 41, p. 335-361, 1987. EVANS, H. J.; EISBRENNER, G.; CANTRELL, M. A.; RUSSELL, S. A.; HANUS, F. J. The present status of hydrogen recycling in legumes. Israel Journal of Botany, Jerusalem, v. 31, n. 1, p. 72-88, 1982. FAGAN, E.B; MEDEIROS, S. L. P.; MANFRON, P.A.; CASAROLI, D.; SIMON, J.; DOURADO NETO, D. ; LIER, Q. J. V.; SANTOS, O. S.; MULLER, L. Fisiologia da fixa??o biol?gica de nitrog?nio em soja. Revista da FZVA. Uruguaiana, v.14, n.1, p. 89-106. 2007. FERREIRA, D. F. Sistema para an?lise de vari?ncia para dados balanceados (SISVAR). Vers?o 4.3. Lavras, Universidade Federal de Lavras, 2003. 37 FREIRE, J. R. J.; VERNETTI, F. J. A. A. A pesquisa com a soja, a sele??o de riz?bio e a produ??o de inoculante no Brasil. Pesquisa Agrop?cuaria Ga?cha, Porto Alegre, V.5, p. 1-25, 1999. FUHRMANN, J. Symbiotic effectiveness of indigenous sobean bradyrhizobia as related to serological, morphological, rhizobitoxine, and hydrogenase phenotypes. Applied and Environmental Microbiology, Washington, v. 56, p. 224-229, 1990. FUJIHARA, S; YAMAMOTO,K; YAMAGUCHI,M. A possible role of allantoin and the influence of nodulation on its production in soybean plants. Plant and Soil, v.48, n.1,p.233-242, 1977. GERAHTY, N.; CAETANO-ANOLL?S, G.; JOSHI, P. A. & GRESSHOFF, P. M. Anatomical analysis of nodule development in soybean reveals an additional autoregulatory control point. Plant Science, v.85, p.1-7, 1992. GUIMAR?ES, A.P. Determina??o do coeficiente isot?pico do 15N relacionado ao processo de fixa??o biol?gica de nitrog?nio em soja 2005. Disserta??o (Mestrado em Agronomia, ?rea de Ci?ncia do Solo) - Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ. GUIMAR?ES, A.P.; DE MORAIS, R.F.; URQUIAGA S.; BODDEY, R.M.; ALVES, B.J.R. Bradyrhizobium strain and the 15N natural abundance quantification of biological N2 fixation in soybean. Scientia Agricola, v. 65, p.516-524, 2008. H?GBERG, P . 15N natural abundance in soil-plant systems. Tansley review n? 95. New Phytologist, v.137, p.179-203, 1997. HOLSTEN, R. D.; BURNS, R. C.; HARD, R. W. F.; HERBERT, R. R. Establisment of Symbiosis between Rhizobium and plant cells in vitro. Nature, London, v. 323, p. 173-175, 1971. HUNGRIA, M. & NEVES, M. C. P. Cultivar and strain effect on nitrogen fixation and transport in Phaseolus vulgaris L. Plant and Soil, 103: 111-121, 1987. HUNGRIA, M.; ANDRADE, D. S.; BALOTA, E. L.; COLOZZI FILHO, A. A import?ncia do sistema de semeadura direta na popula??o microbiana do solo. Londrina: EMBRAPA-CNPSo, 1997. 9 p. (EMBRAPA-CNPSo. Comunicado T?cnico, 56). HUNGRIA, M.; FRANCHINI, J. C.; CAMPO, R. J.; CRISPINO, C. C.; MORAES, Z. J.; SIBALDELLI, R. N. ?R.; MENDES, I. C.; ARIHARA, J. Nitrogen nutrition of soybean in Brazil: Contributions of biological N2 fixation and N fertilizer to grain yield. Canadian journal of plant science, P. 227-239, 2006. HUNGRIA, M.; NEVES, M. C. P.; D?BEREINER, J. Relative efficiency, ureide transport and harvest index in soybeans inoculated with isogenic Hup mutants of Bradyrhizobium japonicum. Biology and Fertility of Soils, Berlin, v. 7, p. 325-329, 1989. JACOB NETO, J.; DUQUE, F. F. An?lise de componentes morfol?gicos de produ??o e fixa??o biol?gica do nitrog?nio em cultivares de soja (Glycine max (L.) Merril). In: SEMIN?RIO NACIONAL DE PESQUISA DE SOJA, 2., 1981, Londrina. Anais... Londrina: EMBRAPA-CNPSo, 1982. v. 2. p. 16-21. JUNK, G.; SVEC, H. J. The absolute abundance of the nitrogen isotopes in the atmosphere and compressed gas from various sources. Geochimica et Cosmochimica Acta, Oxford, v. 14, p. 234-243, 1958. 38 KESSEL, C. VAN; HARTLEY, C. Agricultural management of grain legumes: has it led to an increase in nitrogen fixation. Field Crops Research, V.65, P.165-181, 2000. MACEDO, R. A. T. Influ?ncia de fatores de manejo sobre a fixa??o biol?gica de nitrog?nio na cultura da soja em ?reas experimentais e de produ??o no Noroeste do Paran?. 2003. Disserta??o (Mestrado em Agronomia, ?rea de Ci?ncia do Solo) - Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ. MARIOTTI, A.; MARIOTTI, F.; CHAMPIGNY, M. L.; AMARGER, N.; MOYSE, A. Nitrogen isotope fractionation associated with nitrato reductase activity and uptake of NO3- by pearl millet. Plant Physiology, Rockville, v. 69, p. 880-887, 1982. McCLURE, P.R.; ISRAEL, D.W.; VOLK, R.J. Transport of nitrogen in xylem of soybean plants. Plant Physiology, Rockville, v.66, p. 720-725. 1979. MEEKS, J. C.; WOLK, C. P.; SCHILLING, N.; SHAFFER, P. W.; AVISSAR, Y.; CHIEN, W. S. Initial organic products of fixation of (13N) dinitrogen by root nodules of soybean (Glycine max). Plant Physiology, Rockville, v. 61, p. 980-987, 1978. MENDES, I. C.; J?NIOR, F. B. R.; HUNGRIA, M.; SOUZA, D. M. G.; CAMPO, R. J. Aduba??o nitrogenada suplementar tardia em soja cultivada em latossolos do cerrado. Pesquisa Agropecu?ria Brasileira, V. 43, n. 8 p. 10053-1060, 2008. NELSON, L. M. Hydrogen recycling by Rhizobium leguminosarum isolates and growth and nitrogen contents of pea plants (Pisumum sativum L.). Applied and Environmental Microbiology, 45: 856-861, 1983. NEVES, M. C. P.; DIDONET, A. D.; DUQUE, F. F.; D?BEREINER, J. Rizobium strain effects on nitrogen transport and distribution in soybeans. Journal of Experimental Botany, Oxford, v. 36, p. 1179-1192, 1985. NEVES, M. C. P.; HUNGRIA, M. The physiology of nitrogen fixation in tropical grain legumes. CRC Critical Reviews in Plant Sciences, Boca Raton, v. 6, n. 3, p. 267-321, 1987. NISHI, C. Y. M. Infec??o, competitividade e efici?ncia da FBN em soja inoculada com estirpes de Bradyrhizobium SEMIA 566, SEMIA 586, SEMIA 5079 e SEMIA 5080. 1995. Disserta??o (Mestrado) - Universidade Estadual de Londrina, Londrina, PR. NISHI, C. Y. M.; HUNGRIA, M. Efeito da reinocula??o na soja (Glycine max (L.) Merrill) em um solo com popula??o estabelecida de Bradyrhizobium com as estirpes SEMIA 566, 587, 5019, 5079 e 5080. Pesquisa Agropecu?ria Brasileira, Bras?lia, DF, v. 31, n. 5, p. 359-368, 1996. NORRIS D.O.; TMANNETJE L. The symbiotic specialization of african trifolium spp. in relation to their taxonomy and their agronomic use. east afr. agric. For. J., 29: 214-235, 1964. OKITO, A.;ALVES, B.J.R.;URQUIAGA, S.;BODDEY, R.M. Isotopic fractionation during N2 fixation by four tropical legumes. Soil Biology Biochemistry, Oxford, v.36, p.1179-1190, 2004. PATE, J.S.; ATKINS, C. A.; WHITE, S.T.; RAINBIRD, R.M.; WOO, K.C. Nitrogen nutrition and xylem transport of nitrogen in ureide producing grain legume. Plant Physiology, v.65, p.961-965, 1980. PAUFERRO, N.; GUIMAR?ES, A. P.; JANTALIA, C. P.; URQUIAGA, S.; ALVES, B. J. 39 R.; BODDEY, R. M.;. 15N natural abundance of biologically fixed N2 in soybean is controlled more by the Bradyrhizobium strain than by the variety of the host plant. Soil Biology & Biochemistry, 42 : 1694-1700, 2010. PEOPLES, M. B.; FAIZAH, A. W.; RERKASEM, B.; HERRIDGE, D. F. Methods for evaluating nitrogen fixation by nodulated legumes en the field. Canberra: ACIAR, 1989. PEOPLES, M. B.; TURNER, G. L.; SHAH, Z.; SHAH, S. H.; ASLAM, M.; ALI, S.; MASKEY, S. L.; BHATTARAI, S.; AFANDI, F.; SCHWENKE, G. D.; HERRIDGE, D. F. Evaluation of the 15N natural abundance technique for measuring N2 fixation in experimental plots and farmer?s fields. In: INTERNATIONAL WORKSHOP ON MANAGING LEGUME NITROGEN FIXATION IN CROPPING SYSTEMS OF ASIA, 1997, Hyderabad, ?ndia. Proceedings... ?ndia: ICRISAT, 1997. p. 57-75. Editores: O. P. Rupela, C. Johansen, D. F. Herridge. PERRET, X; STAEHELIN, C. & BROUGHTON W.J. Molecular basis of symbiotic promiscuity. Microbiology and Molecular Biology Reviews, v.64, p.180-201, 2000. RAMOS, M. G.; VILLATORO, M. A. A.; URQUIAGA, S.; ALVES, B. J. R.; BODDEY, R. M. Quantification of the contribution of biological nitrogen fixation to tropical green manure crops and the residual benefit to a subsequent maize crop using 15N-isotope techinques. Journal of Biotechnology, Amsterdam, v. 91, p. 105-115, 2001. REIS, V. M: OLIVEIRA, A. L. M.; BALDANI, V. L. D.; OLIVARES, F. L.; BALDANI, J. I. Fixa??o Biol?gica de Nitrog?nio simbi?tica e associativa: Muito al?m da nutri??o. In: FERNANDES, M. S (ed). Nutri??o Mineral de Plantas. Vi?osa: Sociedade Brasileira de Ci?ncia do Solo, 2006. p. 53 ? 88. RUMJANEK, N. G.; DOBERT, R. C.; VAN BERKUN, P.; TRIPLETT, E. W. Common soybean inoculant strains in Brazil are member of B. Elkanii. Applied and Environmental Microbiology, Washington, v. 59, p. 4371-4371, 1993. RYLE ,G.J. A. et al. The respiratory costs of nitrogen fixation in soyabean, cowpea, and white clover. II. Comparisons of the cost of nitrogen fixation and the utilization of combined nitrogen. Journal of Experimental Botany, v.30, p.145-153, 1979. SANTOS, O. S. dos (Coord.). A cultura da soja?1: Rio Grande do Sul, Santa Catarina, Paran?. Rio de Janeiro: Globo, 1988. 299 p. (Cole??o do Agricultor. Gr?os). SANTOS, V. A. F.; NEVES, M. C. P.; RUMJANECK, N. G. Differential symbiotic efficiency by shading of soybean nodulated by B. Japonicum and B. Elkanii strains. Soil Biology & Biochemistry, Oxford, v. 29, n. 5/6, p. 1015-1018, 1997. SANTOS, V. A. F.; NEVES, M. C. P.; RUMJANECK, N. G. Efficiency of soybean nodules related to Rhizobia hidrogenase is influenced by light level. Revista Brasileira Fisiologia Vegetal, 8(1):15-21, 1996. SHEAER G. E.; KOHL D.H. N2-fixation in field settings: estimations based on natural 15N abundance, Australian Journal of Plant Physiology, Victoria, v,13, p,699-756, 1986. SILVA, A. F.; ET AL. Efeito da inocula??o da soja (Cultivar Tropical) com riz?bios de crescimento r?pido e lento em solo ?cido submetido ? calagem. Acta Scientiarum, V. 24, N. 5, P.1327-1333, 2002. TA?Z, L.; ZIEGER, E. Fisiologia vegetal. 3 ed. Porto Alegre: Artmed, 2004. 40 UNKOVICH, M J. Isotope discrimination provides new insight into biological nitrogen fixation. New Phytologist, v.198, n.3, p.643-646, 2013. UNKOVICH, M. J.; PATE, J. S. An appraisal of recent field measurements of symbiotic N2 fixation by annual legumes. Field Crops Research, Amsterdam, v. 65, p. 211-228, 2000. VALLIS, I. HAYDOCK, K. P., ROSS, P. J., HENZELL, E. F. Isotopic studies on the uptake of nitrogen b y pastures. III. The uptake of small additions of 15N-labelled fertilizer by Rhodes grass and Townsville Lucerne. Australian Journal of Agricultural Research, Melbourne, v. 18, p. 865-877, 1967. VINCENT, G.M. Manual of the practical study of root nodule bacteria. (International Biology Program, 15), Oxford: Blackwell p.163, 1970. WEISBURG, W.G. ; BARNS, S.M; PELLETIER, D.A. AND LANE, DJ. 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology, 173: 697?703, 1991. |
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