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Previous issue date: 2015-02-25 / CAPES / FAPERJ / Instituto Oswaldo Cruz / Previous studies on the helminth fauna of scombrid fish from the Brazilian coast included reports on Scomber japonicus Houttuyn, 1782 and Scomber colias Gmelin, 1789. Nowadays its considered that only S. colias occurs in the Atlantic Ocean, Mediterranean Sea and Black Sea, forming different populations. To assess and revise the parasite biodiversity of S. colias off the coast of Rio de Janeiro State, 69 fish from Public Markets of S?o Pedro da Aldeia and Serop?dica were collected and analyzed, from March 2013 to November 2014. Fish were examined and all organs were individualized in Petri dishes containing saline solution 0.7% to be analyzed under the stereo microscope. The parasites were fixed in 70% etanol, AFA or 4% formalin. The Nematoda were cleared and examined in temporary mounts with glycerin and Monogenea, Trematoda and Cestoda were stained with Semichon's acid carmine or Gomori's trichrome and examined as permanent mounts in Canada balsam. The taxonomic identification was made by measuring with the ocular micrometer, by illustrations using a camera clara, and by studies on scanning electron microscopy and molecular techniques. The prevalence, intensity amplitude, mean abundance and standard deviation were calculated per each parasite species. Parasite community study was made at the infracomunity level using the data of 50 fish collected in a single sample from S?o Pedro da Aldeia, which represents a component community. The mean richness, mean total abundance and the mean of Berger-Parker dominance index, mean Bray-Curtis similarity and the mean of Brillouin diversity index of the infracommunities were also calculated. Additionally, using the SIMPER procedure, were also identified the species that contributed most to the similarity between the infracommunities. We collected 578 parasites belonging to 9 taxa: Monogenea (Grubea cochlear and Kuhnia scombri), Digenea (Aponurus laguncula, Lecithocladium harpodontis and Allonematobothrioides scombri - new host record and new geographical distribution), Cestoda (Scolex pleuronectis) and Nematoda (Raphidascaris sp., Hysterothylacium sp., Anisakidae gen. sp. larvae). Raphidascaris sp. was the most prevalent (64%), followed by K. scombri (60%) and A. laguncula (38%). The most abundant species was K. scombri (2.30% ? 3.73). The mean infracommunity richness was 2.14 ? 1.09 with a total mean abundance of 7.08 ? 7.24. The mean Berger-Parker dominance index was 0.67 ? 0.27, K.scombri, Raphidascaris sp. and A.laguncula being the most dominant species. The Bray-Curtis similarity index between infracommunities was relatively low (32.78 ? 26.20) and Raphidascaris sp. and Hysterothylacium sp. were contributing most to the similarity. / Os trabalhos pr?vios sobre a helmintofauna de peixes escombr?deos da costa brasileira inclu?ram refer?ncias em Scomber japonicus Houttuyn, 1782 e Scomber colias Gmelin, 1789. Hoje se considera que apenas S. colias ocorre no Oceano Atl?ntico, Mar Mediterr?neo e Mar Negro, onde forma popula??es distintas. Com o objetivo de avaliar e revisar a biodiversidade parasit?ria de S. colias, do litoral do Estado do Rio de Janeiro, foram coletados e analisados 69 peixes provenientes de Mercados P?blicos dos Munic?pios de S?o Pedro da Aldeia e Serop?dica, no per?odo de Mar?o de 2013 a Novembro de 2014. Os peixes foram necropsiados e todos os ?rg?os foram individualizados em placas de Petri com solu??o salina 0,7% para serem examinados no microsc?pio estereosc?pico. Os parasitos foram fixados em ?lcool a 70%, AFA ou formalina a 4%. Os Nematoda foram diafanizados e examinados em l?minas tempor?rias com glicerina e os Monogenea, Trematoda e Cestoda corados em Carmim de Semichon ou Tricr?mico de Gomori e examinados em l?minas permanentes montadas em B?lsamo do Canad?. A identifica??o taxon?mica foi feita atrav?s de medidas com aux?lio de ocular microm?trica, desenhos em c?mara clara, estudos por microscopia eletr?nica de varredura e t?cnicas moleculares. Foram calculadas a preval?ncia, amplitude de intensidade, abund?ncia m?dia e desvio padr?o para cada esp?cie de parasito. Os estudos de comunidades se realizaram a n?vel de infracomunidade com os dados de 50 peixes coletados em uma ?nica amostra em S?o Pedro de Aldeia, que representa uma comunidade componente. Foram tamb?m calculadas a riqueza m?dia, a abund?ncia total m?dia, ?ndice m?dio de domin?ncia de Berger-Parker e os ?ndices m?dios de similaridade de Bray-Curtis e de diversidade de Brioullin. Adicionalmente, utilizando o procedimento SIMPER, foram avaliadas as esp?cies que mais contribu?ram para a similaridade entre as infracomunidades. Foram coletados 578 parasitos, pertencentes a 9 taxa: Monogenea (Grubea cochlear e Kuhnia scombri), Digenea (Aponurus laguncula, Lecithocladium harpodontis e Allonematobothrioides scombri ? novo hospedeiro e nova distribui??o geogr?fica), Cestoda (Scolex pleuronectis) e Nematoda (Raphidascaris sp., Hysterothylacium sp., Anisakidae gen. sp. larva). Raphidascaris sp. apresentou maior preval?ncia (64%), seguido por K. scombri (60%) e A. laguncula (38%). A esp?cie mais abundante foi K. scombri (2,30% ? 3,73). A riqueza m?dia de esp?cies a n?vel de infracomunidade foi de 2,14?1,09 com uma abund?ncia total de 7,08?7,24. As infracomunidades apresentaram um ?ndice de domin?ncia de Berger-Parker de 0,67?0,27 sendo as esp?cies mais dominantes K.scombri, Raphidascaris sp. e A.laguncula. O ?ndice de similaridade de Bray-Curtis entre as infracomunidades foi relativamente baixo (32,78?26,20) e as esp?cies Raphidascaris sp. e Hysterothylacium sp. foram as que mais contribu?ram para a similaridade.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:jspui/2293 |
| Date | 25 February 2015 |
| Creators | MORENO, Aline Braga |
| Contributors | Silva, Cl?udia Portes Santos, Silva, Cl?udia Portes Santos, Justo, M?rcia Cristina Nascimento, Alejos, Jos? Luis Fernando Luque |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Programa de P?s-Gradua??o em Ci?ncias Veterin?rias, UFRRJ, Brasil, Instituto de Veterin?ria |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | Portuguese |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
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