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Previous issue date: 2007-02-28 / Funda??o Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro / The purpose of the present study was to evaluate the activity of the neonicotinoid dinotefuran
on the cat flea Ctenocephalides felis felis. Fleas used in controlled and in vitro tests were
obtained from a colony maintained at Laboratory of Development of Parasiticide Products
(LDPP), and the Beagle dogs used for the controlled tests were kept at the same place. Five
concentrations of dinotefuran, 0.2085, 0.417, 0.834, 1.668 and 3.336%, were evaluated for in
vitro activity on eggs and adults of C. felis felis. Dinotefuran did show low activity on flea
eggs, but showed efficacy on adult fleas. Two formulations of dinotefuran were titulated. For
spray formulation, 0.834% was selected and for strip-on formulation, 30% was selected.
Later, the efficacy of two strip-on formulations, one containing 30% dinotefuran and other
with 30% dinotefuran plus 2.575% pyriproxyfen, were evaluated in the control of fleas on
dogs. Dogs were infested with 100 adult fleas and evaluated after 48 hours. The efficacy was
to 99.5 and 100% for day 1 declining to 76.1 and 72.2% on day 35, respectively. Two spray
formulations, one with 0.834% dinotefuran and other with 0.834% dinotefuran plus 0.148%
pyriproxyfen, were tested for the control of fleas on dogs. Dogs were infested and evaluated
after 48 hours. The efficacy was 100% on day 1 for both formulations, reducing for 66.6% on
day 28 and 59.4% on day 35, respectively. Four formulations previously described were
tested on naturally infested housed kept-dogs from Serop?dica City, RJ. Animals were
evaluated weekly. The efficacies were 95.2 on day 7 and 91.4% on day 56 for the spray
formulation with dinotefuran; and 100% on day 7 and 88.1% on day 56 for the spray
association. For dinotefuran strip-on were 98.5% on day 7 and 74% on day 56; and 100% on
day 7 and 81.1% on day 56 for strip-on association. Residual activity of treated dog s hair
with dinotefuran on eggs, larvae and adults of fleas were evaluated. One dog was treated with
0.834% dinotefuran spray, other dog was treated with 30% dinotefuran strip-on and the third
was not treated. Some areas of dog s hair were clipped on days 2, 16, 23, 30, 37 and 44 after
treatment. For the evaluation of adulticidal, larvicidal and ovicidal activities were used adults,
larvae and eggs from the laboratory colony. Six repetitions were used with 10 specimens of
each flea stage per day, placed in assay tubes. In each repetition were added 0.02 g of treated
or untreated dog s hair and larval diet for immature stages. The adulticidal activity was
evaluated during 24 hours, the larvae 20 days after treatment and the eggs 72 after challenge.
The residue on hair of both treated dogs showed efficacy on the larval control for 44 days and
on egg control at day 2. Regarding the adults control the spray and strip-on formulations
showed efficacy until days 16 and 23, respectively. / O presente trabalho teve como objetivo avaliar a atividade do neonicotin?ide dinotefuran
sobre Ctenocephalides felis felis. As pulgas utilizadas nos testes in vitro e nos testes
controlados foram da col?nia mantida no Laborat?rio de Desenvolvimento de Produtos
Parasiticidas (LDPP) da UFRRJ, e os nos testes controlados foram utilizados c?es Beagle
oriundos do canil de experimenta??o mantido nas mesmas depend?ncias. Em um teste in vitro
foram avaliadas as efic?cias de cinco concentra??es de dinotefuran, 0,2085; 0,417; 0,834,
1,668 e 3,336%, sobre ovos e adultos de C. felis felis. O dinotefuran apresentou atividade
parcial sobre os ovos de C. felis felis, por?m se mostrou eficaz no controle de adultos. Duas
formula??es de dinotefuran foram tituladas. Das concentra??es testadas para a formula??o
spray (0,417; 0,834 e 1,668%), a selecionada foi a de 0,834%, e para a formula??o strip-on
(25, 30 e 35%) foi a de 30%. Posteriormente, em um teste controlado, foram avaliadas as
efic?cias de duas formula??es strip-on , uma contendo dinotefuran a 30% e outra o mesmo
associado ao piriproxifen a 2,575%, no controle de adultos de C. felis felis em c?es Beagle. Os
c?es foram infestados semanalmente com 100 pulgas adultas oriundas da col?nia e avaliados
ap?s 48 horas. As efic?cias foram de 99,5 e 100% no dia +1 declinando para 76,1 e 72,2% no
dia +35, respectivamente. Duas formula??es spray, uma contendo dinotefuran a 0,834% e
outra o mesmo associado ao piriproxifen a 0,148%, tamb?m foram avaliadas. As efic?cias
foram de 100% no dia +1 para as duas formula??es, declinando para 66,6% no dia +28 e para
59,4% no dia +35, respectivamente para a formula??o contendo apenas dinotefuran e a
associa??o. As quatro formula??es citadas anteriormente foram testadas em n?vel de campo
em c?es infestados naturalmente domiciliados no Munic?pio de Serop?dica, RJ. Para a
formula??o spray contendo apenas dinotefuran, a efic?cia foi de 95,2% no dia +7 e de 91,4%
no dia +56. J? para formula??o associada, a efic?cia foi de 100% no dia +7 e de 88,1% no dia
+56. Para a formula??o strip-on contendo apenas o dinotefuran, a efic?cia foi de 98,5% no
dia +7 e de 74% no dia +56, e para associa??o a efic?cia foi de 100% no dia +7 e de 81,1% no
dia +49. Foram avaliadas as atividades adulticida, larvicida e ovicida do res?duo no p?lo de
c?es tratados com as formula??es spray e strip-on . Pequenas ?reas do corpo do c?o foram
tricotomizadas nos dias 2, 9, 16, 23, 30, 37 e 44 ap?s o tratamento. Seis repeti??es com 10
exemplares cada, acondicionados em tubos de ensaio, da etapa correspondente foram
utilizados por dia de desafio, e acrescidos de 0,02g de p?lo tratado ou n?o. O res?duo no p?lo
de c?es tratados com ambas as formula??es de dinotefuran foi eficaz no controle de ovos
apenas no dia +2, para larvas por at? 44 dias, e no controle de adultos as formula??es spray e
a strip-on foram eficazes por um per?odo de 16 e 23 dias, respectivamente.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:tede/808 |
| Date | 28 February 2007 |
| Creators | Correia, Tha?s Ribeiro |
| Contributors | Scott, Fabio Barbour, Grisi, Laerte, Famadas, K?tia Maria |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Curso de P?s-Gradua??o em Ci?ncias Veterin?rias, UFRRJ, Brasil, Parasitologia Veterin?ria |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
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