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Previous issue date: 2014-03-26 / Biomphalaria snails act as intermediate hosts that harbor the Schistosoma mansoni parasite, the causative agent of schistosomiasis. As population increases, snails have their geographical distribution conditioned to the presence of calcium in the environment, which is essential for their energetic metabolism. The objective of this study was the assessment on the influence of exposure to different concentrations of CaCO3 (20, 40, 60, 80 and 100 mg/L) at different intervals (1, 14, 21 and 30 days) forthe concentrations of glucose, calcium, total proteins, urea, uric acid and creatinine in hemolymph B. glabrata. All substances were dosed using specific kits with a spectrophotometer reading at 545 nm. High performance liquid chromatography (HPLC) was the technique applied to quantify the presence of organic acids: pyruvate, oxaloacetate, citrate, succinate, fumarate, propionate, acetoacetate and βhydroxybutyrate in the hemolymph of mollusks exposed to CaCO3. Results revealed
that the calcium concentration in the hemolymph did not present significant difference
(p>0.05) with regard to control as well as to the concentrations assessed. The glucose
concentration decreased (p<0.05) in exposures to 20mg and 40mg and increased in
exposures to 80mg and 100mg of CaCO3 with regard to control. The organic acids
pyruvate, oxaloacetate, citrate, succinate fumarate and lactate had their concentrations
increased; propionate in turn had its concentration decreased set against control in
exposure to CaCO3. In the exposures to the concentrations assessed, acetoacetate
decreased and β-hidroxibutirato increased. Total proteins and urea presented decrease.
The uric acid concentration increased in the exposure to 20mg of CaCO3; the creatinine
concentration increased in the exposures to 40mg and 100mg of CaCO3. It was possible
to conclude that snails exposed to different concentrations of CaCO3 had their major
metabolic alterations occurring from the 14th day of exposure. / Moluscos do gênero Biomphalaria são conhecidos pelo seu papel como hospedeiro intermediário do parasito Schistosoma mansoni, causador da doença esquistossomose mansônica. O aumento da população desses moluscos, consequentemente sua distribuição geográfica está condicionada a presença de cálcio no mbiente, também considerado um elemento essencial no metabolismo energético do molusco. Este estudo teve por objetivo avaliar a influência da exposição às diferentes concentrações de CaCO3 (20, 40, 60, 80 e 100 mg/L), em diferentes intervalos de tempo (1, 14, 21 e 30 dias) sobre às concentrações de glicose, cálcio, proteínas totais, ureia, ácido úrico e creatinina na hemolinfa de B. glabrata, ambas as substâncias foram dosadas através de kits específicos e lidas em espectrofotômetro em 545 nm. Através da técnica de cromatografia líquida de alta eficiência (CLAE), foi quantificado a presença de ácidos orgânicos: piruvato, oxaloacetato, citrato, succinato, fumarato, propionato, acetoacetato e β-hidroxibutirato na hemolinfa dos moluscos expostos ao CaCO3. Os resultados demonstraram que a concentração de cálcio na hemolinfa não apresentou diferença
significativa (p>0,05) em relação ao controle, bem como, entre as concentrações testadas. A concentração de glicose diminuiu (p<0,05) nas exposições a 20mg e 40mg e aumentou nas exposições a 80mg e 100mg de CaCO3 em relação ao controle. Os ácidos orgânicos piruvato, oxaloacetato, citrato, succinato, fumarato e lactato tiveram suas concentrações aumentadas e propionato diminuída comparada ao controle na exposição
ao CaCO3. A concentração de acetoacetato diminuiu e a de β-hidroxibutirato aumentou na exposição às concentrações testadas. Houve diminuição na concentração de proteínas totais e de ureia. A concentração de ácido úrico aumentou na exposição a 20mg de CaCO3 e, a concentração de creatinina aumentou nas exposições à 40mg e 100mg de CaCO3. Concluiu-se que moluscos expostos às diferentes concentrações de CaCO3, principalmente a partir de 14 dias, alteram o metabolismo como acontece em situações de estresse fisiológico, com a finalidade de regular o sistema redox do molusco.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.bc.ufg.br:tede/3111 |
| Date | 26 March 2014 |
| Creators | Silva, Luciana Damacena |
| Contributors | Bezerra, José Clecildo Barreto, Castro, Ana Maria de, Mello-Silva, Clélia Christina Corrê de, Bezerra, José Clecildo Barreto, Castro, Ana maria de, Mello-Silva, Clélia Christina Corrêa de, Vinaud, Mariana Clare, Barcelos, Rejane da Silva Sena |
| Publisher | Universidade Federal de Goiás, Programa de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP), UFG, Brasil, Faculdade de Medicina - FM (RG) |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | Portuguese |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFG, instname:Universidade Federal de Goiás, instacron:UFG |
| Rights | http://creativecommons.org/licenses/by-nc-nd/4.0/, info:eu-repo/semantics/openAccess |
| Relation | 6085308344741430434, 600, 600, 600, 1545772475950486338, 2878476940568031721, ABÍLIO, F.J.P.; RUFFO, T.L.M.; SOUZA, A.H.F.F.; FLORENTIN, H.S.; JÚNIOR, E.T.O.; MEIRELES, B.N.; SANTANA, A.C.D. Macroinvertebrados bentônicos como bioindicadores de qualidade ambiental de corpos aquáticos da caatinga Oecologia Brasiliensis, v. 11, n. 3, p. 397-409. 2007. ALLEN, P.J.; D’ANCI, K.E.; KANAREK, R.B. Creatine, brain functioning, and behavior. In: KANAREK, RB.; LIEBERMAN, H. R. Diet, Brain and Behavior: Practical Implications, p. 215-236. 2011. ALP, P.R.; NEWSHOLME, E.A.; ZAMMIT, V.A. Activities of citrate synthase and NADlinked isocitrate dehydrogenase in muscle from vertebrate and invertebrates. Biochemical Journal, v. 154, p. 689-700. 1976. AUGUSTO, R.C.; MAGALHÃES, A.C.S.; MELLO-SILVA, C.C. The influence of population density and food intake on the reproductive biology of Biomphalaria glabrata (Mollusca) and calcium proportion in snails experimentally infected with Schistosoma mansoni (Trematoda), Revista de Patologia Tropical, v. 41, n. 1, p. 83-92. 2012. BARBOSA, F. S.; BARBOSA, S. C. The bioecology of snail vectors for Shistosomiasis in Brazil. Cadernos de Saúde Pública, v.10, n. 2, p. 200-209. 1994. BARBOSA, F.; SIMOES – BARBOSA, C. The bioecology of snail vectors for schistosomiasis in Brazil. Cadernos de Saúde Pública, v.10, n.02, p.200-209. 1994. BARKER, G.M. Mollusks as Crop Pests CABI Publishing, Walling-ford, Oxon, UK. 2002. 441p. BÉARD, E.; BRAISSANT, O. Synthesis and transport of creatine in the CNS: importance for cerebral functions. Journal of Neurochemistry, v. 115, p. 297–313. 2010. BECKER, W. Metabolic interrelation ships of parasitic trematodes and mollusks, especially Schistosoma mansoni in Biomphalaria glabrata. Z Parasitenkd, v. 63, p.101-111. 1980. BECKER , W. Purine metabolism in Biomphalaria glabrata under starvation and infection with Schistosoma mansoni. Comparative Biochemistry and Physiology, v. 76B, p. 215-9. 1983. BECKER, W.; LΫTH, W. Der Einfluβ Von Hunger und infection MIT Schistosoma mansoni auf den ketonkorpergehalt der hamolymphe Von Biomphalaria glabrata. Z. Parasitenkd, v. 53, p. 109-113. 1977. BEIS, A.; ZAMMIT, V.A.; NEWSHOLME, E.A. Activities of 3-hydroxybutyrate dehydrogenase, 3-oxo-acid CoA-transferase and acetoacetyl-CoA-thiolase in relation to ketone body utilisation in muscles from vertebrates and invertebrates. European Journal of Biochemistry, v. 104, n. 209-215. 1980. BEZERRA, J.C.B.; BECKER, W.; ZELCK, U.E. A comparative study of the organic acid content of the hemolymph of Schistosoma mansoni-resistant and susceptible strains of Biomphalaria glabrata. Memórias do Instituto Oswaldo Cruz, v. 92, n.3, p. 421-425. 1997. BEZERRA, J.C.B.; KEMPER, A.; BECKER, W. Profile of organic acid concentrations in the digestive gland and hemolymph of Biomphalaria glabrata under estivation. Memórias do Instituto Oswaldo Cruz, v. 94, p. 779-784. 1999. BISHOP, M.L.; DUBEN-ENGELKIRK, J.L.; FODY, E.P. Clinical chemistry. Principles, procedures, correlations, thirdth edn. Lippincott, Filadélfia. 1996. BRIERS, R.A. Range size and environmental calcium requirements of British freshwater gastropods. Global Ecolology and Biogeography, v.12, p. 47-51. 2003. BURTIS, C.A.; ASHWOOD, E.R. Textbook of clinical chemistry, 2nd Philadelphia: W.B. Sauders, p. 2175- 2211. 1986. CACECI, T.; NECK, K.; LEWIS, D.; SIS, R.F. Ultrastructure of the hepatopancreas of the pacific white shrimp, Penaeus vannamei (Crustacea: Decapoda). Journal of the Marine Biological Association of the United Kingdom, v. 68, p. 323-337. 1988. CAMPBELL, G.; JONES, C.S.; LOKYER, A.E.; HUGHES, S.; BROWN, D.; NOBLE, L.R.; ROLLINSON, D. Molecular evidence supports an African affinity of the Neotropical glabrata Say, 1818, an intermediate host for Schistosoma mansoni. Proceedings of the Royal Society of London, v. 267, p. 2351–2358. 2000. CARVALHO, O.S.; ROCHA, R.S.; MASSARA, C.L.; KATZ, N. Primeiros casos autóctones de esquistossomose mansonica em região do noroeste do Estado de Minas Gerais (Brasil). Revista de Saúde Pública, v. 22, p. 237–239. 1988. CIAN, M.C.; REGNAULT, M.; LALLIER, F.H. Nitrogen metabolites and related enzymatic activities in the body fluids and tissues of the hydrothermal vent tubeworm Riftia Pachyptila. Journal of Experimental Biology, v. 203, p. 2907–2920. 2000. CONNERTY, H.; BRIGGS, A.R.; EATEN, E.H. Determination of blood urea nitrogen using a simple stabilizing reagent. American Journal of Clinical Pathology, v. 25, n.1321. 1955. DALESMAN, S.; BRAUN, M.H.; LUKOWIAK, K. Low environmental calcium blocks long term memory formation in a pulmonate snail. Neurobiology of Learning Memory, v. 95, p. 393-403. 2011. DAWIES, T.W.; ERASMUS, D.A. An ultrastructural study of the effect of parasitism by larval Schistosoma mansoni on the calcium reserves of host, Biomphalaria globata. Cell Tissue Research, v. 236, p. 643-649. 1984. DE JONG, R.J.; MORGAN, J.A.T.; PARAENSE, W.L.; POINTIER, J.P.; AMARISTA, M.; AYEH-KUMI, P.F.K.; BABIKER, A.; BARBOSA, C.S.; BRE´MOND, P.; CANESE, A.P.; PEREIRA DE SOUZA, C.; DOMINGUEZ, C.; FILE, S.; GUTIE´RREZ, A.; INCANI, R.N.; KAWANO, T.; KAZIBWE, F.; KPIKPI, J.; LWAMBO, N.J.S.; MIMPFOUNDI, R.; NJIOKOU, F.; PODA, J.N.; SENE, M.; VELA´SQUEZ, L.E.; YONG, M.; ADEMA, C.M.; HOFKIN, B.V.; MKOJI, G.M.; LOKER, E.S. Evolutionary relationships and biogeography of Biomphalaria (Gastropoda: Planorbidae) with implications regarding its role as host of the human bloodfluke, Schistosoma mansoni. Molecular Biology and Evolution, v. 18, p. 2225– 2239. 2001 HOFKIN, B.V.; MKOJI, G.M.; LOKER, E.S. Evolutionary relationships and biogeography of Biomphalaria (Gastropoda: Planorbidae) with implications regarding its role as host of the human bloodfluke, Schistosoma mansoni. Molecular Biology and Evolution, v. 18, p. 2225– 2239. 2001. DEJONG, R.J.; MORGAN, J.A.T.; WILSON, W.D, AL-JASER, M.H.; APPLETON, C.C.; COULIBALY, G.; D’ANDREA, P.S.; DOENHOFF, M.J.; HAAS, W.; IDRIS, M.A.; MAGALHÃES, L.A.; MONE´ H.; MOUAHID, G.; MUBILA, L.; POINTIER, J.P.; WEBSTER, J.P.; ZANOTTI-MAGALHÃES, E.M.; PARAENSE, W.L.; MKOJI, G.M.; LOKER, E.S. Phylogeography of Biomphalaria glabrata and B. pfeifferi, important intermediate hosts of Schistosoma mansoni in the New and Old World tropics. Molecular Ecology, v. 12, p. 3041–3056. 2003. DOERING, C.J.; ZAMPONI, G. Molecular Pharmacology of High Voltage-Activated Calcium Channels. Journal of Bioenergetics and Biomembranes, v. 35, n. 6, p 210-213. 2003. EBANKS ,S.C.; O’DONNELL, M.J.; GROSELL, M. Characterization of mechanisms for Ca2+ and HCO3–/CO32– acquisition for Shell formation in embryos of the freshwater common pond snail Lymnaea stagnalis. The Journal of Experimental Biology, v. 213, p. 4092-4098. 2010. FARO, M.J.; PERAZZINI, M.; CORRÊA, L.R.; MELLO-SILVA, C.C.; PINHEIRO, J.; MOTA, E.M.; SOUZA, S.; ANDRADE, Z.; JÚNIOR, A.M. Biological, biochemical and histopathological features related to parasitic Greenaway P. Calcium regulation in the freshwater mollusc, Limnaea stagnalis (L.) (Gastropoda: Pulmonata). 1. The effect of internal and external calcium concentration. The Journal of Experimental Biology, v. 54, p. 199214. 2013. GUIMARÃES, R.J.P.S.; FREITAS, C.C.; DUTRA, L.V.; FELGUEIRAS, C.A.; MOURAD, A.C.M.; AMARAL, R.S.; DRUMMONDF, S.C.; SCHOLTEA, R.G.C.; OLIVEIRA, G.; CARVALHO, O.S. Spatial distribution of Biomphalaria mollusks at São Francisco River Basin, Minas Gerais, Brazil, using geostatistical procedures. Acta Tropica, v. 109, p. 181– 186. 2009. HENRY, J.B. Clinical diagnosis and management by laboratory methods, 19nd, 1996. p. 164-165. HIONG, K.C.; LOONG, A.M.; CHEW, S.F.; KWONG, Y.I. Increases in urea synthesis and the ornithine–urea cycle capacity in the giant african snail, Achatina fulica, during fasting or a estivation, or after the injection with ammonium chloride. Journal of Experimental Zoology, v. 303A, p.1040–1053. 2005. HOCHACHKA, P.V. 1983. In: WILBUR, J. M (ed) The Mollusca. Vol 1, Academic Press, New York: 510p. 1983. JOKINEN, E.H. The freswater snails of Concecticut. State of Connecticut Geological and Natural History Survey, department of Environmental Protection. Bulletin, v. 109: n. 83. 1983. LEHNINGER, A. L. Princípios de Bioquímica. São Paulo. 4nd ed. Sarvier, 2006. p. 606615. LEWIS B.R.; JUTTNER, I.; REYNOLDS, B.; ORMEROD, S.J. Comparative assessment of stream acidity using diatoms and macroinvertebrates: implications for river management and conservation. Aquat. Conserv.: Mar. Freshwater Ecosystem, v. 17, p. 502–519. 2007. LIE, K.L.; HEYNEMAN, D. Studies on resistence in snails. Escape of Echinostoma lindoense sporocysts from encapsulation in the snail heart and subsequent loss of the host’s ability to resist infection by the same parasite. Journal of Parasitology, v. 62, n. 298-302. 1976. LIEBSCH, M.; BECKER, W. Comparative glucose tolerance studies in the freshwater snail Biomphalaria glabrata: influence of starvation and infection with the trematode Schistosoma mansoni. . Journal of Comparative Physiol B, v. 160, p. 41-50. 1990. LIVINGSTONE, D.R.; DE ZWAAN, A. Carbohydrate metabolism of gastropods. In: Hochachka PW. The Mollusca. Metabolic Biochemistry and Molecular Biomechanics. USA: Academis Press, v1, p. 177-242. 1983. MACHADO, J.; COIMBRA, J.; SA, C.; CARDOSO, I. Shell thickening in Anodonta cygnea by induced acidosis. . Comparative Biochemistry and Physiology, v. 91, p. 645–651. 1988. MADSEN, H.; FRANDSEN, F. The spread of freshwater snails including those of medical and veterinary importance. Acta Tropica, v. 46, p. 139–146. 1989. MADSEN, H. Ecology and control of Africans freshwater pulmonate snails. 1985. 117p. MAGALHÃES, A.C.; PINHEIRO, J.; MELLO-SILVA, C.C. A mobilização do cálcio em Biomphalaria glabrata exposta a diferentes quantidades de carbonato de cálcio. Revista de Patologia Tropical, v. 40, n.1, p. 46-55. 2011a. MAGALHÃES, A.C.; AUGUSTO, R.C.; MELLO-SILVA, C.C. Biomphalaria glabrata: Exposure to different amounts of calcium carbonat e influencing growth, sexual mat uration and pearl’s formation. Revista de Patologia Tropical, v. 40, n. 4, p. 341-347. 2011b. MARIN, F.; LUQUET, G. Molluscan shell proteins. C. R. Comptes Rendus Paleontology, v. 3, p. 469-492. 2004. MARXEN, J.C.; BECKER, W. Calcium binding constituents of the organic shell matrix from the freshwater snail Biomphalaria glabrata. Comparative Biochemistry and Physiology, v. 127 B, p. 235–242. 2000. MARXEN, J.C.; NIMTZ, M.; BECKER, W.; MANN, K. The major soluble 19.6 kDa protein of the organic shell matrix of the freshwater snail Biomphalaria glabrata is na Nglycosylated dermatopontin. Biochemistry and Biophysica Acta, v. 1650, p. 92– 98. 2003. MASSA, D.R.; MICHAEL, J.; FRIED, C.B.; JOSEPH, S, J. High performance column liquid chromatographic analysis of selected carboxylic acids in Biomphalaria glabrata patently infected with Schistosoma mansoni. Parasitology Research, v. 101, p. 925–928. 2007. MATRICON-GONDRAN, M. The site of ultrafiltration in the kidney saco f the pulmonate gastropod Biomphalaria glabrata. Tissue Cell, v. 22, p. 911-923. 1990. MELLO-SILVA, C.C. Controle Alternativo e Alterações Fisiológicas em Biomphalaria glabrata (Say, 1818), Hospedeiro Intermediário de Schistosoma mansoni Sambom, 1907 pela Ação do Látex de Euphorbia splendens var. hislopii N.E.B (Euphorbiaceae). 2005. 85f.. Tese - niversidade Federal Rural do Rio de Janeiro, Seropédica. 2005. MELLO-SILVA, C.C.; VASCONCELLOS, M.C.; PINHEIRO, J.; RODRIGUES, M.L. A Physiological changes in Biomphalaria glabrata Say, 1818 (Pulmonata: Planorbidae) caused by sub-lethal concentrations of the látex of euphorbia splendens var. hislopii N. E. B (Euphorbiaceae). Memórias do Instituto Oswaldo Cruz, v. 10, n.1, p. 3-8. 2006. MELLO-SILVA, C.C.; VILAR, M.M.; VASCONCELLOS, M.C.; PINHEIRO, J.; RODRIGUES, M.L.A. Carbohydrate metabolism alterations in Biomphalaria glabrata infected with Schistosoma mansoni and exposed to Euphorbia splendens var. hislopii látex. Memórias do Instituto Oswaldo Cruz, v. 4, p. 492-495. 2010. MEYER, R.; BECKER, W.; KLIMKEWITZ, M. Investigations of the ketone body metabolism of Biomphalaria glabrata: Influence of starvation and infection with Schistosoma mansoni. Journal Comparative Physiology, v. 156B, p. 563-567. 1986. MISKIN, E.M.; JOKINEN, E.H. Effects of environmental calcium on fecundity and cercarial production of Biomphalaria glabrata (SAY) infected with Schistosoma mansoni Sambon. Journal of Parasitology, v. 72, n. 6, p. 885-890. 1986. MOREIRA, L.E. Moluscos Moreira LE, 5nd. Ed. da UCG, Goiânia .cap. 5, p. 151-155: In Curso Programada de Paleontologia Geral e de Invertebrados. 2004. MOTTA, V.T. Metabolismo do glicogênio e gliconeogênese. In: Bioquímica. p.147. 2005. NAIRAN, A.S. The amebocytes of lamellibranch mollusk with special reference to the circulating amoebocytes. Malacological Review, v. 6, p. 1-12. 1973. PARAENSE, W.L. Estudo atual da sistemática dos planorbídeos brasileiros. Revista Brasileira de Zoologia, v. 55, p. 105-128. 1975. PARAENSE, W.L. Planorbídeos hospedeiros intermediários do Schistosoma mansoni. In: CUNHA, A.S. Esquistossomose Mansoni: Por um grupo de colaboradores especializados. São Paulo, Sarvier/Edups.Cap. II, 1970. p. 13-30. PERRY, S.F.; SHAHSAVARANI, A.; GEORGALIS, T.; BAYAA, M.; FURIMSKY, M.; THOMA, S.S.L.Y. Channels, pumps, and exchangers in the gill and kidney of freshwater fishes: Their role in ionic and acid-base regulation. Journal of Experimental Zoology, v. 300ª, p. 53-62. 2003. PIERI, O.S. Aspectos Ecológicos. In: Brasil. Ministério da Saúde. Secretaria de Vigilância em saúde. Departamento de Vigilância Epidemiológica. Vigilância e controle de moluscos de importância epidemiológica: Diretrizes técnicas: Programa de Vigilância controle da esquistossomose (PCE). - 2. Ed. –Brasília :Editora do Ministério da Saúde, v. 3, 2007. p. 37-40. PIERI, O.S.; RAYMUNDO, J.S; JURBERG, P. Estudos sobre o comportamento dos planorbídeos: II- Enterramento de Biomphalaria glabrata como meio de proteção contra a dessecação. Memórias do Instituto Oswaldo Cruz, v. 75, n. ½, p. 83-88. 1980. PINHEIRO, J. Influence of starvation on the glycogen and galactogen content in the snail Bradybaena similaris (Férussac, 1821) (Mollusca, Gastropodo). Arquivos de Biologia e Tecnologia, v. 39, n. 2, p. 349-357. 1996. PINHEIRO, J.; GOMES, E.M.; CHAGAS, G.M. Aminotransferases activity in the hemolymph of Bradybaena similaris (Gastropoda, Xanthonychidae) under Starvation. Memórias do Instituto Oswaldo Cruz, v. 96, n. 8, p. 1161-1164. 2001. PYNNONEN, K. Accumulation of Ca in the freshwater unionids Anodonta anatina and Unio tumidus, as influenced by water hardness, protons, and aluminum. Journal of Experimental Zoology, v. 260, p. 18–27. 1991. ROSA, E. Observações ecológicas sobre Biomphalaria straminea (Dunker, 1848) em áreas do nordeste, Brasil. Memória do Instituto Oswaldo Cruz, v. 82, n. 4, p. 311-314. 1987. RUDDELL, C.L.; WELLINGS, S.R. The fine structure of granular amoebocytes of the Pacific oyster, Crassostrea gigas. Jounal of invertebrate Pathology, v. 18, p. 269-275. 1971. RUNDLE, S.D.; SPICER, J. I.; COLEMAN, R. A.; VOSPER, J.; SOANE, J. Environmental calcium modifies induced defences in snails. Proceeding of the Royal Society, v. 271, p. 6770. 2004. RUPPRECHT, H.; BECKER, W.; SCHAWANBEK, A. Alterations in hemolymph components in Biomphalaria glabrata during long-term infection with Schistosoma mansoni. Parasitology Research, v. 75, p.233-237. 1989. SANTOS, M.A.V.; BRABO, E.S.; CARNEIRO, B.S.; FAIAL, K.F.; RODRIGUES, I.R.C. Estudo quantitativo de metais presentes na hemolinfa de Biomphalaria glabrata (Gastropoda), infectadas e não infectadas com Schistosoma mansoni. Revista da Sociedade Brasileira de Medicina Tropical, v. 38, n. 2, p. 157-160. 2005. SILVA, M.J.M.; BARROS, M. Occurrence and Distribution of Fresh-Water Molluscs in the Riacho Fundo Creek Basin, Brasilia, Brazil. Revista Brasileira de Biologia v.49, n. 3-4, p. 865-870. 2001. SIMKISS, K.; MASON, A.Z. Metal ions: Metabolic and toxic effects. In: HOCHACHKA, P. W. The Mollusca. Academic Press, New York, cap.4, 1982. p. 137. SOUZA, C.P.; DRUMOND, S.C.; SILVA, C.J.E.; QUEIROZ, L.A.; GUIMARÃES, C.T.; ROCHA, R.S. Investigação sobre a transmissão da esquistossomose no complexo turístico da Serra do Cipó, MG. Iesus, v. 7, n. 4, p. 43-51. 1998. SOUZA, C.P.; CLARK, L. Moluscos de interesse parasitológico do Brasil. 2nd. Fiocruz Belo Horizonte, 1997. 73p. THOMAS, J. D.; EATON, P. The origins, fate, and ecological significance of free amino compounds released by freshwater pulmonate snails. Comparative Biochemistry and Physiology, v. 119A, n. 1, p. 341–349. 1998. TIELENS, A.G.M. Energy generation in parasitic helminths. Parasitology Today, v. 10, p. 346–352. 1994. TUNHOLI, V.M.; LUSTRINO, D.; TUNHOLI-ALVES, V.M.; MELLO-SILVA, C.C.C.; MALDONADO-JÚNIOR.; RODRIGUES, M.L.A.; PINHEIRO, J. Changes in the reproductive biology of Biomphalara glabrata infected with different doses of Echinostoma paraensei miracidia. Journal of Invertebrate Pathology, v. 106, p. 192–195. 2011. TUNHOLI, V.M.; TUNHOLI-ALVES, V.M.; LUSTRINO, D.; ROSANE, N.; CASTRO, R.N.; SANT’ANA, L.O.; GARCIA, J.S.; ARNALDO, M.A.J.; SANTOS, M.A.J.; RODRIGUES, M.L.A.; PINHEIRO, J. Aerobic to anaerobic transition in Biomphalaria glabrata (Say, 1818) infected with different miracidial doses of Echinostoma paraensei (Lie and Basch, 1967) by high-performance liquid chromatography. Experimental Parasitology, v. 133, p. 403–410. 2013. TUNHOLI-ALVES, V.M.; TUNHOLI, V.M.; GARCIA, J.S.; COSTA-NETO, S.F.; MALDONADO-JÚNIOR, A.; SANTOS, M.A.J.; THIENGO, S.C.; PINHEIRO, J. Changes in the calcium metabolism of Biomphalaria glabrata experimentally infected with Angiostrongylus cantonensis. Journal of Helminthology, p. 1- 6. 2012. VINAUD, M.C.2007. Vias do metabolismo energético e respiratório em cisticercos de Taenia crassiceps in vivo e seu estudo in vitro sob a ação de fármacos anti-helmínticos. 2007. 107f. Tese – Universidade Federal de Goiás. 2007. WEICHSELBAUM, C.T.E. An accurate and rapid method for determination of proteins in small amounts of blood serum and plasma. American Journal of Clinical Pathology, 16, p. 40–49. 1946. YOUNG, D.S. Effects of preanalytical variables on clinical laboratory tests. 1nd, Washinsgton: AACC Press. 1993. ZARAI, Z.; BOULAIS, N.; KARRAY, A.; MISERY, L.; BEZZINE, S.T.; REBAI, T.; GARGOURI1, Y.; MEJDOUB, H. Immunohistochemical localization of hepatopancreatic phospholipase A2 in Hexaplex Trunculus digestive cells. Lipids in Health and Disease, v. 10, n. 91, p. 1-9. 2011. ZELCK, U.E.; BECKER, W.; BAYNE, C.J. The plasma proteins of Biomphalaria glabrata in the presence and absence of Schistosoma mansoni. Developmental and Comparative Immunology, v. 19, p. 181-194. 1995. |
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