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Previous issue date: 2015-12-15 / FACEPE / Os Papilomavírus humano (HPV) infectam mucosas e epitélio contribuindo
para o desenvolvimento de tumores benignos como também malignos. São
amplamente conhecidos como causadores do câncer cervical, contudo,
atualmente, vem apresentando evidências de associação com diversos outros
tipos de canceres, como o câncer de pulmão. Sendo assim, o presente estudo
avaliou a presença do DNA do HPV em tumores de pulmão de pacientes do
Estado de Pernambuco bem como a expressão de suas oncoproteínas E6 e E7.
Para isto, a detecção foi feita em amostras de tecidos de tumores frescos e
parafinados de 63 pacientes. HPV estava presente em 52% das amostras, sendo
detectados os tipos 16 e 18 com frequências de 81 e 19%, respectivamente.
Quanto a presença do vírus nos diferentes tipos histológicos dos tumores, foi
detectado HPV em 40% dos carcinomas escamosos, 33% dos adenocarcinomas,
18% dos carcinomas de células pequenas e 9% em carcinoma de células
grandes. Através da técnica de imunohistoquimica detectou-se a presença das
oncoproteinas virais E6 (anticorpo anti-HPV 16 e anti-HPV 18) e E7 (anticorpo
anti-HPV 16 e anti-HPV 18) com frequências de 85 e 75%, respectivamente. Tal
resultado confirma os resultados obtidos molecularmente quanto à presença do
HPV e é sugestivo de que o vírus esteja em atividade nas células tumorais e
provavelmente esteja desempenhando um papel na carcinogênese de pulmão. No
entanto, mais estudos são necessários para se ter um maior esclarecimento sobre
interação de E6 e E7 com proteínas celulares na tumorigenese pulmonar. / Small DNA viruses - Human Papillomavirus (HPV) - infect oral mucosa and
the epthelium, which leads to the development of both benign and malign tumors.
They are widely known as the principal causes of cervical cancer although
currently there is evidence to show that they are associated with several other
types of cancer, such as lung cancer. In the light of this, this study evaluated the
presence of HPV in the tumors of lungs of patients from the State of Pernambuco,
as well as the E6 and E7 oncoproteins expression. This involved carrying out the
detection in tumor tissue samples that were fresh and paraffin-embedded and
taken from 63 patients. HPV was found to be present in 52% of the samples, and
types 16 and 18 were detected with frequencies of 81% and 19% respectively.
With regard to the presence of the vírus in different histological types of tumors,
HPV was detected in 40% of the squamous carcinomas, 33% of the
adenocarcinomas, 18% of the small cell carcinomas and 9% in large cell
carcinomas. The presence of the E6 (antibody anti-HPV 16 and anti-HPV 18) and
E7 (antibody anti-HPV 16 and anti-HPV 18) oncoproteins was detected by means
of the immunohistochemical technique and this confirmed the results obtained
from a molecular analysis with regard to the presence of the virus and it is
suggestive that the virus is active in tumor cells and is probably playing a role in
lung carcinogenesis. However, further studies are required to have a clearer
understanding of the interaction of E6 and E7 with the cell proteins in pulmonary
tumorigenesis.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufpe.br:123456789/18464 |
| Date | 15 December 2015 |
| Creators | AMARAL, Carolina Maria Medeiros Do |
| Contributors | FREITAS, Antonio Carlos de, NETO, Jacinto da Costa Silva |
| Publisher | Universidade Federal de Pernambuco, Programa de Pos Graduacao em Genetica, UFPE, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Breton |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Source | reponame:Repositório Institucional da UFPE, instname:Universidade Federal de Pernambuco, instacron:UFPE |
| Rights | Attribution-NonCommercial-NoDerivs 3.0 Brazil, http://creativecommons.org/licenses/by-nc-nd/3.0/br/, info:eu-repo/semantics/openAccess |
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