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Previous issue date: 2012-02-27 / The expression of human leukocyte antigen G (HLA-G) and human leukocyte antigen E (HLA-E) in physiological and pathological processes remains unknown, it is believed that these molecules play a fundamental role in the establishment and maintenance of immune tolerance by inhibiting the functions of immunocompetent cells. In literature we found no published study involving the bacterium Helicobacter pylori (H. pylori) with expression of HLA-G and HLA-E. The objective this study is investigated the expression of this protein in gastric biopsies of patients with the bacterium H. pylori. Sixty-four biopsies of the patients with diagnosis of infection by H. pylori were evaluated to expression of HLA-G and HLA-E. The samples were stratified according to the presence of carcinoma or peptic ulcers. Patients without H. pylori were used to control. To investigate the expression of this protein were used immunohistochemistry technique with monoclonal antibody anti-HLA-G and anti-HLA-E. Other criteria such as analysis of the inflammatory infiltrate (hematoxylin-eosin) and identification of H. pylori (Giemsa) were analyzed. We detected HLA-G and HLA-E molecules in the most samples containing ulcer and gastric carcinoma. In negative control group was not detected the presence of HLA-G and HLA-E. The presence of H. pylori seems modulate the expression of HLA-G and HLA-E, favoring the evolution of infection, giving different degrees of gastric lesion in epithelium of these patients / A express?o do ant?geno leucocit?rio humano G (HLA-G) e do ant?geno leucocit?rio humano E (HLA-E) em processos fisiol?gicos e patol?gicos permanece pouco conhecida. Acredita-se que essas mol?culas desempenham papel fundamental no estabelecimento e manuten??o da toler?ncia imunol?gica, inibindo as fun??es das c?lulas imunocompetentes. Na literatura internacional, at? o momento, n?o foi encontrado nenhum estudo publicado correlacionando Helicobacter pylori (H. pylori) com express?o de HLA-G e HLA-E. O presente trabalho tem como objetivo correlacionar a express?o dessas prote?nas em bi?psias g?stricas de pacientes acometidos com H. pylori. Sessenta e quatro esp?cimes g?stricas de pacientes acometidos com H. pylori foram avaliados para express?o de HLA-G e HLA-E. As amostras foram estratificadas de acordo com a presen?a de carcinoma ou de ?lcera p?ptica. Como controle foram analisados esp?cimes g?stricas de pacientes vivos sem H. pylori. Para detectar a express?o dessas mol?culas utilizou-se a t?cnica de imunohistoqu?mica com os anticorpos monoclonais anti-HLA-G e anti-HLA-E. Outros crit?rios como an?lise do infiltrado inflamat?rio (hematoxilina-eosina) e identifica??o do H. pylori (Giemsa) foram analisados. As mol?culas de HLA-G e HLA-E foram detectadas em grande parte das amostras contendo ?lcera e carcinoma g?strico. No grupo controle n?o foi detectada a presen?a de HLA-G e HLA-E, indicando que a bact?ria H. pylori modula a express?o dessas mol?culas no grupo dos pacientes que apresentaram ?lcera ou carcinoma g?strico. A presen?a da bact?ria H. pylori parece modular a express?o do HLA-G e do HLA-E, favorecendo assim a evolu??o da infec??o, o que confere diferentes graus de les?o do epit?lio g?strico desses pacientes
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/13484 |
| Date | 27 February 2012 |
| Creators | Souza, Daliana Maria Berenice de O |
| Contributors | CPF:91430364491, http://lattes.cnpq.br/2644540835478572, Soares, Edson Garcia, CPF:53512197868, http://lattes.cnpq.br/9338770260143276, Lima, Francisco Pignataro, CPF:44340400491, http://lattes.cnpq.br/5776408959536754, Freitas, Janaina Cristiana de Oliveira Crispim |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Ci?ncias Farmac?uticas, UFRN, BR, Bioan?lises e Medicamentos |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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