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Previous issue date: 2011-02-04 / In the last years, many scientific researches in implantology have been focused
on alternatives that would provide higher speed and quality in the process of
osseointegration. Different treatment methods can be used to modify the
topographic and chemical properties of titanium surface in order to optimize the
tissue-implant reactions by a positive tissue response. This study aimed to
evaluate the adhesion and proliferation of mesenchymal cells from human
periodontal ligament on two different titanium surfaces, using cell culture
techniques. Grade II titanium discs received different surface treatments,
forming two distinct groups: polished and cathodic cage plasma nitriding.
Human periodontal ligament mesenchymal cells were cultured on titanium discs
in 24-well cell culture plates, at a density of 2 x 104 cells per well, including wells
with no discs as positive control. Data obtained by counting the cells that
adhered to the titanium surfaces (polished group and cathodic cage group) and
to the plastic surface (control group), in the 24, 48 and 72-hour periods after
plating, were used to analyze cell adhesion and proliferation and to obtain the
cell growing curve in the different groups. The data were submitted to nonparametric
analysis and the differences between groups were compared by
Kruskal-Wallis and Friedman statistical tests. No statistically significant
differences were found in the cells counts between the groups (p>0.05). It was
concluded that both treatments produced surfaces compatible with the adhesion
and proliferation of human periodontal ligament mesenchymal cells / Nos ?ltimos anos, v?rias pesquisas cient?ficas em Implantodontia t?m buscado
alternativas que proporcionem maior rapidez e qualidade no processo de
osseointegra??o. Diferentes m?todos de tratamento podem ser utilizados para
modificar as propriedades topogr?ficas e qu?micas da sua superf?cie do tit?nio,
a fim de otimizar as rea??es tecido-implante atrav?s de uma resposta tecidual
favor?vel. O presente trabalho teve como objetivo analisar a ades?o e
prolifera??o de c?lulas mesenquimais de ligamento periodontal humano a
diferentes superf?cies de tit?nio, atrav?s de t?cnicas de cultivo celular. Discos
de tit?nio grau II receberam diferentes tratamentos de superf?cie, constituindo
dois grupos distintos: polido e nitretado a plasma na configura??o de gaiola
cat?dica. C?lulas mesenquimais obtidas do ligamento periodontal de dentes
humanos h?gidos foram cultivadas sobre os discos de tit?nio em placas de
cultivo de 24 po?os, na densidade de 2 x 104 c?lulas/po?o, incluindo po?os sem
discos como controle positivo. Os dados obtidos das contagens das c?lulas
aderidas ?s superf?cies de tit?nio (grupo polido e grupo gaiola cat?dica) e ?
superf?cie pl?stica (grupo controle), nos intervalos de 24, 48 e 72 horas ap?s o
plaqueamento, foram utilizados para analisar a ades?o e prolifera??o celular e
obter a curva de crescimento celular nos diferentes grupos. Os dados foram
submetidos a an?lises n?o param?tricas e as diferen?as entre os grupos foram
comparadas pelos testes estat?sticos Kruskal-Wallis e Friedman. N?o foram
encontradas diferen?as estat?sticas significativas nas contagens celulares entre
os grupos estudados (p>0,05). Conclui-se que ambos os tratamentos
produziram superf?cies compat?veis com ades?o e prolifera??o de c?lulas
mesenquimais do ligamento periodontal humano
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/17065 |
| Date | 04 February 2011 |
| Creators | Ribeiro, Rodrigo Alves |
| Contributors | CPF:67269621420, http://lattes.cnpq.br/5004397230198722, Gurgel, Bruno C?sar de Vasconcelos, CPF:02569549410, http://lattes.cnpq.br/4649601602612601, Godoy, Gustavo Pina, CPF:85762997472, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4751939A2, Barbosa, Carlos Augusto Galv?o |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Odontologia, UFRN, BR, Odontologia Preventiva e Social; Periodontia e Pr?tese Dent?ria |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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