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Previous issue date: 2013-03-22 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq / C?es s?o os reservat?rios prim?rios dos parasitos do g?nero Leishmania. A
resposta imune induzida pela infec??o por Leishmania infantum nestes animais n?o est?
completamente elucidada, e poucos estudos tem investigado a correla??o entre a express?o de
citocinas e quimiocinas e as manifesta??es cl?nicas dos animais portadores de leishmaniose
visceral canina (LVC). O objetivo deste estudo foi correlacionar os achados cl?nico em c?es
naturalmente infectados por L. infantum (das ?reas rurais do munic?pio de Mossor?, Estado do
Rio Grande do Norte, Brasil) com os n?veis de express?o de citocinas (IFN-?, TNF-?, IL-10 e
IL-17), iNOS, quimiocinas (CCL1, CCL2, CCL3, CCL4 , CCL5, CCL17, CCL20, CCL24,
CCL26, CXCL9, CXCL10), receptores de quimiocinas (CXCR3, CCR3, CCR4, CCR5,
CCR6, CCR8), e parasitismo no f?gado e no ba?o. Inicialmente, foi realizado levantamento
soroepidemiol?gico de c?es de 12 assentamentos rurais de Mossor?-RN, totalizando 398 c?es
examinados. A positividade dos c?es para o parasito foi determinada pela reatividade em dois
testes sorol?gicos (RIFI e ELISA). Todas as localidades rurais apresentaram c?es positivos
para L. infantum, com positividade variando entre 6,6% a 49% dos animais examinados por
localidade, e um total de 27% de c?es de todas as localidades. Posteriormente, vinte e um c?es
foram clinicamente avaliados e classificados como assintom?ticos (n = 11) ou sintom?ticos (n
= 10). A infec??o foi confirmada pela realiza??o de imprint no ba?o, e observa??o de formas
amastigotas. Esplenomegalia, perda de peso e onicogrifose foram os sinais cl?nicos mais
frequentes. No f?gado, os n?veis de express?o de RNAm de citocinas (IFN-?, TNF-?, IL-10 e
IL-17), iNOS e quimiocinas (CCL1, CCL17, CCL26, CCR3, CCR4, CCR5, CCR6, e CCR8)
foram mais baixos nos animais sintom?ticos do que nos animais assintom?ticos. Em
compara??o com os animais n?o infectados, c?es sintom?ticos obtiveram n?veis mais baixos
de express?o de quase todas as mol?culas analisadas. Al?m disso, foi observada alta carga
parasit?ria e baixa inflama??o hep?tica em animais sintom?ticos, quando comparado aos
assintom?ticos. Conclui-se que a diminui??o da express?o de citocinas, quimiocinas e
receptores de quimiocinas resultaria na migra??o e ativa??o deficiente de leuc?citos,
dificultando o controle do parasitismo e conduzindo ao desenvolvimento da doen?a. / Dogs are the primary reservoirs of the parasites of the genus Leishmania. The
immune response induced by infection with Leishmania infantum in these animals is not
completely understood, and few studies have investigated the correlation between the
expression of cytokines and chemokines and clinical manifestations of animals with canine
visceral leishmaniasis (CVL). The aim of this study was to correlate the clinical status of dogs
naturally infected with L. infantum (rural municipality of Mossoro, Rio Grande do Norte,
Brazil) with the expression levels of cytokines (IFN-?, TNF-?, IL-10 and IL-17), iNOS,
chemokines (CCL1, CCL2, CCL3, CCL4, CCL5, CCL17, CCL20, CCL24, CCL26, CXCL9,
CXCL10), chemokine receptors (CXCR3, CCR3, CCR4, CCR5, CCR6, CCR8), and
parasitism in the liver and spleen using real-time PCR. Initially, we conducted
seroepidemiological survey of dogs from 12 rural settlements from Mossor?-RN, totaling 398
dogs examined. The positivity of dogs for the parasite was determined by reativity in two
serological tests (ELISA and IFA). All rural localities showed dogs positive for L. infantum,
with positivity varying between 6,6% and 49% of the animals examined by location,
totalizing 27% of dogs from all settlements. Subsequently, Twenty-one dogs were clinically
evaluated and classified as asymptomatic (n = 11) or symptomatic (n = 10). The infection was
confirmed by performing imprint in the spleen, and observation of amastigotes.
Splenomegaly, weight loss and onychogryphosis were the most frequent clinical signs. In the
liver, levels of mRNA expression of cytokines (IFN-?, TNF-?, IL-10 and IL-17), iNOS and
chemokines (CCL1, CCL17, CCL26, CCR3, CCR4, CCR5, CCR6, CCR8 and) were lower in
symptomatic animals than in asymptomatic animals. In comparison with uninfected animals,
symptomatic dogs had lower levels of expression of almost all molecules tested. In addition,
the parasitic load was observed high and low symptomatic hepatic inflammation in animals
when compared to asymptomatic. In conclusion, the decreased expression of cytokines,
chemokines and chemokine receptors result in poor migration and activation of leukocytes,
hindering control of parasitism and leading to the development of the disease.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/19725 |
| Date | 22 March 2013 |
| Creators | Albuquerque, Talyta Delfino Rolim de |
| Contributors | 02351506731, http://lattes.cnpq.br/5445410844075357, Andrade Neto, Valter Ferreira de, 59626305487, http://lattes.cnpq.br/4863082845974813, Silva, Andr? Talvani Pedrosa da, 97061697687, Guedes, Paulo Marcos da Matta |
| Publisher | Universidade Federal do Rio Grande do Norte, PROGRAMA DE P?S-GRADUA??O EM CI?NCIAS BIOL?GICAS, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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