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Previous issue date: 2015-07-24 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Acidentes notificados envolvendo o envenenamento por escorpi?es ainda s?o frequentes no Brasil, ocasionados principalmente pela esp?cie Tityus serrulatus, conhecido como escorpi?o amarelo. Embora os soros antiescorpi?nicos sejam produzidos rotineiramente por diversos laborat?rios oficiais, a efic?cia de sua utiliza??o depende da rapidez com que se inicia o tratamento e da efici?ncia na produ??o de anticorpos pelos animais imunizados. No presente trabalho, o desenvolvimento de nanopart?culas polim?ricas cati?nicas de poli(?cido l?tico) teve como objetivo a busca de um sistema de libera??o modificada para os pept?deos e prote?nas presentes na pe?onha do escorpi?o T. serrulatus, capaz de potencializar a produ??o de anticorpos em soro anti-veneno. As nanopart?culas cati?nicas foram obtidas por nanoprecipita??o, ap?s o estudo do efeito dos par?metros da t?cnica sobre as propriedades f?sico-qu?micas das part?culas, com a otimiza??o de um m?todo de baixa energia. A funcionaliza??o da superf?cie das nanopart?culas com a polietilenoimina hiper-ramificada foi comprovada pela an?lise de potencial zeta e possibilitou a adsor??o por intera??o eletrost?ticade diferentes tipos de prote?nas. A efici?ncia de incorpora??o de 40-80 % de albumina de sorobovino (BSA) e 100 % de pept?deos da pe?onha do escorpi?o avaliadas por espectrofotometria e eletroforese em gel de poliacrilamida, confirmou o sucesso na escolha dos par?metros do m?todo de obten??o das nanopart?culas, produzidas com tamanho entre 100 a 250 nm. A an?lise de microscopia de for?a at?mica e estudos in vitro de libera??o, mostraram que as nanopart?culas esf?ricas fornecem um perfil sustentado de libera??o das prote?nas pelo mecanismo de difus?o, que potencializou a produ??o de anticorpos antiveneno em animais imunizados com as nanopart?culas contendo a pe?onha do escorpi?o, comprovando o potencial para aplica??o in vivo das nanopart?culas. / Reported accidents involving the poisoning scorpions are still frequent in Brazil, mainly
caused by Tityus serrulatus, known as yellow scorpion. Although antivenom sera are
produced routinely by various government laboratories, the effectiveness of its use depends
on how quickly treatment is initiated and efficiency in the production of antibodies by the
immunized animals. In this study, the development of cationic polymeric nanoparticles of
poly(lactic acid) aimed to create a modified delivery system for peptides and proteins of T.
serrulatus venom, able to enhance the production of serum antibodies against the scorpion
toxins. The cationic nanoparticles were obtained by a low energy nanoprecipitation, after
study of the parameters? variations effects over the physicochemical properties of the
particles. The surface functionalization of the nanoparticles with the hyperbranched
polyethyleneimine was proved by zeta potential analysis and enabled the adsorption by
electrostatic interaction of different types of proteins. The protein loading efficiency of 40-80
% to bovine serum albumin (BSA) and 100 % to scorpion venom peptides evaluated by
spectrophotometry and polyacrylamide gel electrophoresis confirmed the success of the
selected parameters established for obtainment of nanoparticles, produced with size between
100 to 250 nm. The atomic force microscopy analysis and in vitro release showed that the
spherical nanoparticles provided a sustained release profile of proteins by diffusion
mechanism, demonstrating the potential for application of the nanoparticles in vivo.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/20941 |
| Date | 24 July 2015 |
| Creators | Mesquita, Philippe de Castro |
| Contributors | 00840406452, http://lattes.cnpq.br/2593509584288129, Luchessi, Andr? Ducati, 30489740839, http://lattes.cnpq.br/4420863418928278, Azevedo, Ricardo Bentes de, 15454746215, http://lattes.cnpq.br/9072332484355315, Pedrosa, Matheus de Freitas Fernandes, Silva J?nior, Arnobio Antonio da |
| Publisher | Universidade Federal do Rio Grande do Norte, PROGRAMA DE P?S-GRADUA??O EM CI?NCIAS FARMACEUTICAS, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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