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Previous issue date: 2016-11-17 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / O emprego de plantas medicinais para o tratamento, cura e preven??o de patologias
? um h?bito que acompanha a humanidade desde seus prim?rdios. Considerando que
o Brasil apresenta uma grande biodiversidade para a produ??o de fitoter?picos, ? de
suma import?ncia o estudo de plantas como fontes alternativas de tratamento, visando
a busca de susbt?ncias que possam auxiliar os f?rmacos atualmente prescritos na
terap?utica cl?nica. Durante muitos anos, doen?as cardiovasculares e doen?as
tromboemb?licas tem sido as principais causas de morte por doen?as no mundo,
sendo respons?veis pela morte de cerca de 17,5 milh?es de pessoas at? o ano de
2012 (31% das mortes em todo o mundo). O sistema de coagula??o est? centralmente
envolvido na forma??o do trombo venoso. Indica??es atualmente definidas para
anticoagulantes incluem a profilaxia e tratamento do tromboembolismo. Heparinas
n?o fracionadas e heparinas de baixa massa molar s?o utilizadas como medicamentos
anticoagulantes, no entanto, estes compostos s?o acompanhados de alguns efeitos
secund?rios, tais como, trombocitopenia e um elevado risco de hemorragia. A efeito
deste problema tem se gerado uma procura de novas subst?ncias, a fim de auxiliar a
terap?utica anticoagulante. Neste contexto, este estudo teve como objetivo avaliar o
potencial anticoagulante, efeitos t?xicos e hemorr?gicos dos extratos foliares de
Licania rigida Benth e Turnera subulata, esp?cies vegetais amplamente encontradas
no semi?rido nordestino. Os extratos foram obtidos a partir de etanol (50%), com
posterior parti??o com solventes de polaridades crescentes, incluindo hexano e
acetato de etila. A avalia??o dos extratos frente ao sistema de coagula??o mostrou
uma atividade anticoagulante satisfat?ria pelo Tempo de Tromboplastina parcial
Ativada e Tempo de Protrombina (100% de atividade), atividade Anti-Xa (~ 40% de
inibi??o) e uma grande capacidade de inibibir diretamente da trombina (~ 80 a 100%
de inibi??o) como principal mecanismo de a??o. Al?m disso, observou-se que os
extratos apresentam um baixo efeito hemorr?gico, bem como, a aus?ncia de
toxicidade em modelos in vitro (Citotoxicidade por MTT) e in vivo (Toxicidade oral
aguda). Este trabalho relata pela primeira vez o potencial anticoagulante de Licania
rigida Benth e Turnera subulata. / The use of medicinal plants for the treatment, cure and prevention of pathologies is a
habit that accompanies humanity since its beginnings. Considering that Brazil presents
a great biodiversity for the production of phytotherapics, it is of paramount importance
the study of plants as alternative sources of treatment, aiming at the search for
substances that may help the drugs currently prescribed in clinical therapeutics. Over
many years, cardiovascular disease and thromboembolic disorders have been the
leading cause of death by disease in the world, being responsible for the death of
approximately 17.5 million people by the year 2012 (31% of deaths worldwide). The
coagulation system is centrally involved in the formation of venous thrombus. Currently
defined indications for anticoagulants include prophylaxis and treatment of
thromboembolism. Unfractionated heparins and low molecular heparins are used as
anticoagulant drugs. However, these compounds are accompanied by several side
effects such as thrombocytopenia and a high risk of systemic bleeding. The effect of
this problem demanded the search for new substances in order to assist prolonged
anticoagulant therapy. In this context, this study aimed to evaluate the anticoagulant
potential, toxic and hemorrhagic effects from Licania rigida Benth and Turnera subulata
leaves, species widely found in Northeast semiarid. The extracts were obtained from
ethanol (50%) with subsequent partition with solvents of increasing polarities, including
hexane and ethyl acetate.The crude extracts were obtained from ethanol (50%) and
subsequent partition with increasingly polar solvents including hexane and ethyl
acetate. The evaluation of the extracts against the coagulation system showed a
satisfactory anticoagulant activity by Activated Partial Thromboplastin Time and
Prothrombin Time (100% activity), Anti-Xa activity (~ 40% inhibition) and a large
capacity to inhibit directly from Thrombin (~ 80 to 100% inhibition) as the main
mechanism of action. In addition, the extracts were found to have a low hemorrhagic
effect, as well as the absence of toxicity in in vitro models (MTT cytotoxicity) and in
vivo (acute oral toxicity). This paper reports for the first time the anticoagulant potential
of Licania rigida Benth and Turnera subulata.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/22371 |
| Date | 17 November 2016 |
| Creators | Luz, Jefferson Rom?rio Duarte da |
| Contributors | 20054548420, Langassner, Silvana Maria Zucolotto, 81995725072, http://lattes.cnpq.br/7390416147619446, Dore, Celina Maria Pinto Guerra, 03383860418, http://lattes.cnpq.br/2279068301048550, Rocha, Hugo Alexandre de Oliveira, Thornton, Maria das Gra?as Almeida |
| Publisher | PROGRAMA DE P?S-GRADUA??O EM CI?NCIAS DA SA?DE, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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