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Previous issue date: 2017-05-19 / A otite externa canina ? uma enfermidade relevante na cl?nica veterin?ria. Considerando a elevada frequ?ncia de atendimentos de c?es com otite externa bacteriana, objetivou-se identificar a frequ?ncia etiol?gica da otopatia e tra?ar o perfil de resist?ncia dos Staphylococcus Meticilina Resistentes (SMR) e enterobact?rias produtoras de Beta-Lactamases de Espectro Estendido (ESBL) em pacientes ambulatoriais e de internamento. As amostras foram submetidas a isolamento prim?rio, testes de susceptibilidade ? antimicrobianos e de resist?ncia bacteriana. Para os Staphylococcus sp. aplicou-se o Teste D (Disk Test) e o teste de Resist?ncia ? Meticilina (Met-25). Nas cepas positivas para o Met-25 foi feita Rea??o em Cadeia da Polimerase (PCR) para detec??o do gene mecA. Para estirpes positivas na PCR foi determinada a Concentra??o Bactericida M?nima (CBM) do gluconato de clorexidina. Os grupos bacterianos isolados das 140 amostras coletadas foram: Staphylococcus sp. (122 ? 49,38%), Pseudomonas sp. (22 ? 8,91%), enterobact?rias (19 ? 7,69%), Enterococcus sp. (17 ? 6,88%) e Acinetobacter sp. (12 ? 4,86%). Foram isoladas 14 cepas de SMR (11,47%) e todas continham o gene mecA. Observou-se que 14 Staphylococcus sp. foram Teste D positivo. Para os Staphylococcus coagulase positiva (SCP) a CBM foi de 500.000?g/mL (0,5%) enquanto que para os Staphylococcus coagulase negativa (SCN) o valor da CBM foi de 62.500?g/mL (0,0625%). Detectou-se 3 cepas de enterobact?rias ESBL. Os antimicrobianos mais efetivos para Staphylococcus sp. foram: Linezolida (100%), Rifampicina (94,26%) e Cloranfenicol (92,62%). Para Pseudomonas sp.: Cefepime (100%), Imipenem (100%) e Meropenem (100%). Para as enterobact?rias: Ertapenem (100%), Imipenem (100%) e Cefotaxima (84,21%). Para Enterococcus sp.: Cloranfenicol (100%), Linezolida (94,12%) e Vancomicina (88,24%). Para Acinetobacter sp.: Imipenem (100%), Meropenem (100%) e Ticarcilina com Clavulanato (91,67%). Apesar do gene mecA e de enterobact?rias produtoras de ESBL terem baixa ocorr?ncia neste estudo, existe a necessidade de um monitoramento constante em cepas isoladas de animais dom?sticos. Cultura bacteriol?gica e os testes de suscetibilidade aos agentes antimicrobianos s?o de grande import?ncia na rotina cl?nica veterin?ria para garantir o sucesso do tratamento de infec??es otol?gicas caninas. / The canine external otitis is a relevant disease regarding veterinary clinic. Considering the high frequency of treatment of dogs with external bacteria otitis, this research aims to identify the etiological frequency of otopathy and trace the resistance profile of the Methicillin Resistant Staphylococcus (MRS) and enterobacteria that produce Extended Spectrum Beta-Lactamase (ESBL) in outpatients and internment patients. The samples were submitted to primary isolation, tests of antimicrobial susceptibility and bacterial resistance. For the Staphylococcus sp. the Disk Test was applied along with the resistance test to Methicillin (Met-25). In the positive strains for Met-25, a Polimerase Chain Reaction (PCR) was applied to detect the mecA gene. For the PCR positive strains, a Minimum Bactericidal Concentration (MBC) of chlorhexidine gluconate was determined. The isolated bacterial groups of the 140 samples collected were: Staphylococcus sp. (122 ? 49.38%), Pseudomonas sp. (22 ? 8.91%), enterobacteria (19 ? 7.69%), Enterococcus sp. (17 ? 6.88%) and Acinetobacter sp. (12 ? 4.86%). 14 MRS strains (11.47%) were observed and all off them had the mecA gene. 14 Staphylococcus sp. were Disk Test positive. Concerning the positive Staphylococcus coagulase, the MBC was 500.00?g/mL (0.5%) whereas for the negative Staphylococcus coagulase the MBC value was 62.500?g/mL (0.0625%). 3 ESBL enterobacteria strains were detected. The more effective antimicrobial for the Staphylococcus sp. were: Linezolid (100%), Rifampicin (94.26%) and Chloramphenicol (92.62%). Regarding the Pseudomonas sp.: Cefepime (100%), Imipenem (100%) and Meropenem (100%). Regarding the enterobacteria: Ertapenem (100%), Imipenem (100%) and Cefotaxime (84.21%). Regarding the Enterococcus sp.: Chloramphenicol (100%), Linezolid (94.12%) and Vancomycin (88.24%). Regarding the Acinetobacter sp.: Imipenem (100%), Meropenem (100%) and Ticarcillin with Clavulanate (91.67%). Although the mecA gene and the enterobacteria that produce ESBL are briefly presented in this research, there is a need for constant monitoring of isolated strains in domestic animals. The bacteriological growth and the susceptibility tests in the antimicrobial agents are of great importance in the veterinary clinic routine in order to insure the success in treating canine otological infections.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/24747 |
| Date | 19 May 2017 |
| Creators | Carvalho, Leonardo C?sar Andriola |
| Contributors | 46132252304, Gama, Renata Antonaci, 03893282602, Porto, Wagnner Jos? Nascimento, 02447915462, Motta Neto, Renato |
| Publisher | PROGRAMA DE P?S-GRADUA??O EM CI?NCIAS BIOL?GICAS, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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