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000784203.pdf: 2707585 bytes, checksum: 744cb15abdfa0446e7f2731acecf8660 (MD5) / Infecções por nematódeos gastrintestinais causam consideráveis perdas na pecuária. Haemonchus spp. é o principal nematódeo que acometem bovinos e ovinos principalmente em regiões tropicais e subtropicais. Portanto este estudo teve por objetivo avaliar metodologias de diagnóstico baseado em analises morfológicas e moleculares de larvas infectantes e adultos de Haemonchus spp. Foram utilizados 23 bezerros e 20 cordeiros naturalmente infectados por nematódeos gastrintestinais. Antes da necropsia dos animais, fezes foram coletadas para realização de coproculturas e obtenção de larvas infectantes, que foram morfometricamente analisadas. Logo após o sacrifício dos animais, os abomasos foram removidos e congelados, para posterior recuperação dos exemplares de Haemonchus spp. que foram armazenados em álcool 70º. No caso dos ovinos, os parasitas permaneceram estocados em alcool 70º por mais de um ano antes de serem analisados, enquanto que os parasitas dos bovinos foram armazenados por apenas quatro meses. O diagnóstico morfológico dos Haemonchus machos foi realizado por meio da análise de sínlofe e mensuração de espículos. Os mesmos parasitas utilizados na análise morfológica foram submetidos à análise molecular, com utilização da PCR, com os primers 52/154, 75/86 e Hplacbotu. A técnica padrão ouro para identificação de Haemonchus similis foi análise de espículos e para Haemonchus placei foi PCR com os primes HplacBotu. Todas as amostras de ovinos foram consideradas como sendo de Haemonchus contortus, devido à especificidade parasitária e nenhuma evidencia de outras espécies de Haemonchus nas análises moleculares. Os resultados demonstraram diferença estatística no comprimento da distância entre o final da L3 e o final da bainha das larvas infectantes das três espécies. Houve sobreposição de medidas de apenas 1,26% entre as espécies H. placei e H. contortus. Na análises moleculares o par ... / Nematodes gastrointestinal infection causes considerable losses in the livestock industry. Haemonchus spp. is major nematode that affects cattle and sheep mainly in tropical and subtropical region. Therefore, the aim of the present study was to assess diagnosis methodologies based in morphological and molecular analysis of infective larvae and adults of Haemonchus spp. Twenty-three calves and twenty lambs naturally infected with gastrointestinal nematodes were utilized. Before necropsy of animals, faecal samples were collated for preparing faecal cultures and obtaining infective larvae that were morphometrically analyzed. After the euthanasia of animals the abomasums were removed and frozen for posterior recovered Haemonchus specimens that were stored in alcohol 70º. In the case sheep, the parasites remained stored in alcohol 70º for more than one year before be analyzed, while the parasites of cattle were stored for only four months. The morphological diagnosis of males Haemonchus spp. was released by synlophe analysis and spicule measurements. The same parasites used in the morphological analysis were submitted in the molecular analysis, using PCR, with primers 52/159, 75/86 and HplacBotu. The “gold standard” method used to identify Haemonchus similis was spicules analysis and to identify Haemonchus placei was PCR with the primers HplacBotu. All Haemonchus specimens recovered from sheep were assumed to be Haemonchus contortus based on host specificity, and because the molecular analysis did not show any evidence of other Haemonchus species. The results presented statistic differences in the sheath tail length of infective larvae of three species. There was overlapping of measurements was only 1.26% between H.contortus and H. placei. In the espicule analysis also were observed same overlapping of measurements that caused misidentification of H. placei and H. contortus. In relation the synlophe most samples ...
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.unesp.br:11449/111041 |
| Date | 28 February 2014 |
| Creators | Silva, Maria Regina Lucas da [UNESP] |
| Contributors | Universidade Estadual Paulista (UNESP), Amarante, Alessandro Francisco Talamini do [UNESP] |
| Publisher | Universidade Estadual Paulista (UNESP) |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | 56 f. |
| Source | Aleph, reponame:Repositório Institucional da UNESP, instname:Universidade Estadual Paulista, instacron:UNESP |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -1, -1 |
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