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Previous issue date: 2014-02-24 / The PLGA is a bioabsorbable polymer derived from lactic and glycolic acids which has been used as drug release system . One of the drugs with the potential to use this type of vehicle is the recombinant human growth hormone (rhGH), because it has advantages such as speed up the bone integration process , bone maturation and remodeling around implants, in addition to stimulating osteogenesis. Objective: This study aims to evaluate the matrix degradation of PLGA and rhGH.
Materials and Methods: 75:25 (Group I) and 50:50 (Group II): Two ratios of PLGA were used. The matrices were prepared by solvent evaporation technique as it follows: firstly, the PLGA was dissolved in dichloromethane and methanol and then it was mixed with a composed mixture of recombinant human growth hormone, zinc acetate, solution and deionized water. This mixture was poured into silicone circular shape moulds approximately 10 mm diameter which were placed in a solvent evaporation chamber at room temperature, where they were dried by nitrogen gas force action. The control samples were made just dissolving PLGA and pouring it into the silicone moulds. After the drying period, all the samples were weighed on a precision scale. Then, they were immersed in buffer solution and the hydrolytic degradation experiment was investigated and the samples remained for a predetermined period of time. After this process was finished, the solid sample portion was dried and weighed again. The supernatant went through pH analysis and later, by UV-visible absortion spectrophotometry analysis. Results:It was possible to calculate the concentration of rhGH released in the middle through the straight line obtained in a calibration curve.We observed that, from the first to the last day the released rhGH concentration in the medium was higher in Group II. The loss of mass percentage increased over the time in both groups. But this loss of mass was higher in group I compared to Group II. The pH showed a little variation over the time. The average was between 7.5 and 7.9. Conclusion:The matrices which were prepared with PLGA 50:50 showed the best results in relation to the ones prepared with PLGA 75:75.These are some advantages: easy handling, more uniform pores, less bubbles and the growth hormone was released slowly and gradually. / O PLGA ? um pol?mero bioabsorv?vel derivado dos ?cidos l?ctico e glic?lico que tem sido usado como sistema de libera??o de drogas. Uma das drogas com potencial de utiliza??o nesse tipo de ve?culo ? o horm?nio do crescimento humano recombinante (rhGH), pois apresenta vantagens como, por exemplo, acelerar o processo de osteointegra??o, remodela??o ?ssea e matura??o ao redor de implantes, al?m de estimular a osteog?nese. Objetivo: Este trabalho tem por objetivo avaliar a degrada??o de matrizes de PLGA com rhGH. Materiais e M?todos: Foram utilizadas duas propor??es de PLGA: 75:25 (Grupo I) e 50:50 (Grupo II). As matrizes foram confeccionadas por meio da t?cnica de evapora??o de solventes da seguinte forma: primeiramente o PLGA foi dissolvido com diclorometano e metanol e, posteriormente, foi misturado ? uma solu??o composta por horm?nio do crescimento humano recombinante, acetato de zinco e ?gua deionizada. A mistura dessas subst?ncias foi vertida em moldes de silicone de formato circular, com aproximadamente 10 mm de di?metro, os quais foram acondicionados em uma c?mara de evapora??o de solvente, ? temperatura ambiente, onde as matrizes foram secas por meio do arraste for?ado pela a??o de g?s nitrog?nio. As amostras controle, entretanto, foram confeccionadas apenas dissolvendo o PLGA e vertendo-o nos moldes de silicone. Ap?s o per?odo de secagem todas as amostras foram pesadas em uma balan?a de precis?o. Na sequ?ncia, as matrizes foram imersas em solu??o tamp?o e realizou-se o ensaio de degrada??o hidrol?tica, no qual as amostras permaneceram por um per?odo de tempo pr?-determinado. Terminado esse processo, a parte s?lida da amostra foi seca e, novamente, pesada. O sobrenadante, ent?o, passou pela an?lise de pH e pela an?lise de espectrofotometria de absor??o UV-vis?vel. Resultados: Atrav?s da equa??o da reta obtida em uma curva de calibra??o, foi poss?vel calcular a concentra??o do rhGH liberado no meio. Com isso, observou-se que, do primeiro at? o ?ltimo dia a concentra??o de rhGH liberado no meio foi maior no Grupo II. O percentual de perda de massa foi crescente ao longo tempo, em ambos os grupos. Por?m essa perda de massa foi maior no Grupo I, em rela??o ao Grupo II. O pH apresentou pouca varia??o ao longo do tempo. A m?dia ficou entre 7,5 e 7,9. Conclus?o: As matrizes preparadas com PLGA na propor??o 50:50 apresentaram melhores resultados em rela??o ?s matrizes preparadas com PLGA 75:25. Dentre as vantagens, ressaltam-se: maior facilidade de manipula??o, poros mais uniformes, menos bolhas de ar e libera??o do horm?nio do crescimento de forma mais lenta e gradual.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/1267 |
| Date | 24 February 2014 |
| Creators | Duarte, Aline Adelaide Paz da Silva |
| Contributors | Pagnoncelli, Rogerio Miranda |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Odontologia, PUCRS, BR, Faculdade de Odontologia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -8096554818733665164, 500, 600, 4673435736271820140 |
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