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Previous issue date: 2015-01-23 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The objective of the present study was to evaluate the effects of an antiinfective
surgical therapy over 20 peri-implant crevicular fluid biomarkers in
patients with peri-implantitis and to compare these immunologic profiles with the
clinical parameters.
Fifty-two patients presenting implants affected by peri-implantitis were
classified into 4 groups according with probing depth and the presence of
bleeding and/or suppuration at mesio-buccal site of each implant with periimplantitis.
Clinical parameters were measured and PICF samples were
collected from the mesio-buccal site of every implant. All subjects were
submitted to anti-infective surgical therapy. Clinical parameters and PICF
biomarkers were re-measured at 6 and 12 months after treatment. PICF
samples were analyzed using multiplex bead immunoassay for 20 biomarkers.
Differences over time were analyzed using generalized estimating equations.
The significance level established for all analyses was 5% (p <0.05).
The results demonstrated a significant reduction of the clinical
parameters post-therapy. However, it was observed a significant increase of
some pro-inflammatory biomarkers levels (IL-1?, IL-6, IL-12p40, IL-12p70,
IL-17a e sCD-40L). And IL-13 was the only anti-inflammatory cytokine, which
had a significant increase in its levels after anti-infective surgical therapy. / O presente estudo tem por objetivo avaliar o efeito da terapia cir?rgica
anti-infecciosa sobre 20 biomarcadores imunol?gicos em pacientes com periimplantite,
e comparar esse perfil imunol?gico com os par?metros cl?nicos.
Foram selecionados 52 pacientes que apresentavam implantes com
peri-implantite, os quais foram divididos em 4 grupos de acordo com a
profundidade de sondagem e presen?a de sangramento e/ou supura??o no
s?tio m?sio-vestibular de cada implante com peri-implantite. Os par?metros
cl?nicos foram mensurados e amostras de fluido crevicular peri-implantar,
coletadas do s?tio m?sio-vestibular de cada implante. Todos pacientes foram
submetidos ? terapia cir?rgica anti-infecciosa e reavaliados 6 e 12 meses ap?s
o tratamento. As amostras de fluido crevicular foram analisadas atrav?s do
ensaio imunol?gico Luminex (multi-an?lise com micro-esferas) para detec??o
de 20 biomarcadores. Diferen?as entre os grupos em rela??o aos n?veis desses
biomarcadores ao longo do tempo foram analisadas estatisticamente atrav?s
das equa??es de estima??o generalizadas. O n?vel de signific?ncia
estabelecido foi de 5% (p < 0,05).
Os resultados demonstraram redu??es significativas dos par?metros
cl?nicos, entretanto mostraram aumentos significativos dos n?veis de alguns
biomarcadores pr?-inflamat?rios (IL-1?, IL-6, IL-12p40, IL-12p70, IL-17a e
sCD-40L) ap?s a terapia cir?rgica. A IL-13 foi a ?nica citocina anti-inflamat?ria
que mostrou aumento significativo ap?s o tratamento.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/6136 |
| Date | 23 January 2015 |
| Creators | Zani, Sabrina Rebollo |
| Contributors | Teixeira, Eduardo Rolim, Teles, Ricardo Palmier |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Odontologia, PUCRS, Brasil, Faculdade de Odontologia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -8096554818733665164, 600, 600, 600, 600, 4673435736271820140, -2070498469879244349, 2075167498588264571 |
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