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Efeito imunomodulador de diferentes extratos de Angiostrongylus cantonensis no desenvolvimento de resposta pulmonar al?rgica em um modelo murino

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Previous issue date: 2017-03-31 / Asthma is a chronic inflammatory disease of the airways, associated with genetic and environmental factors. Exposure to helminths appears to exert a protective effect on some chronic diseases, such as asthma. The objective of this study was to evaluate the immunomodulatory effect of different extracts of Angiostrongylus cantonensis (A. cantonensis) on the development of allergic pulmonary response by ovalbumin (OVA) in mice. The animals were divided into 6 groups: negative control (PBS), positive control (OVA), animals with asthma immunized with total soluble extract of A. cantonensis (Ac/OVA), animals with proteins from the digestive tract (AcD/OVA), animals with proteins from the reproductive tract (AcR /OVA), and animals with proteins from the cuticle (AcC / OVA). At the end of the OVA protocol, the animals were euthanized for blood collection, bronchoalveolar lavage and lung tissue. OVA-specific IgE was analyzed in blood, and cell counts, IL-4, IL-5, IL-10, INF- ? and eotaxin in bronchoalveolar lavage. Histological analysis of the lung was also performed. A significant reduction in the total cell count was observed in the groups that were immunized with the total soluble extract (TE) and with the soluble extract of the systems (SE) of A. cantonensis, compared to the OVA group (p <0.001). We found a significant reduction of eosinophils, macrophages and lymphocytes in the group immunized with TE, as in the SE groups, in relation to the OVA group. However, intraperitoneal immunization with TE and SE did not alter the levels of OVA-specific IgE and cytokines, except for an increase in IL-10 and IFN-? (p <0.01) in the group immunized with TE. In conclusion, the different worm extracts from A. cantonensis, especially TE, when administered early intraperitoneally, result in a modulation of the OVA-induced allergic lung response in mice, with a distinct response of IL-10 and e INF-? in the TE group. / A asma ? uma doen?a inflamat?ria cr?nica das vias a?reas, associada ? fatores gen?ticos e ambientais. A exposi??o a helmintos parece exercer um efeito protetor sobre algumas doen?as cr?nicas, como a asma. O objetivo deste estudo foi avaliar o efeito imunomodulador de diferentes extratos de Angiostrongylus cantonensis (A. cantonensis) no desenvolvimento da resposta pulmonar al?rgica por ovalbumina (OVA) em camundongos. Os animais foram divididos em 6 grupos: controle negativo (PBS), controle positivo (OVA), animais com asma imunizados com extrato total sol?vel de A. cantonensis (Ac/OVA), com prote?nas do aparelho digestivo (AcD/OVA), com prote?nas do aparelho reprodutor (AcR/OVA), e de prote?nas da cut?cula (AcC/OVA). No final do protocolo de OVA, os animais foram eutanasiados para coleta de sangue, lavado broncoalveolar e tecido pulmonar. Foram dosados IgE espec?fica para OVA no sangue, e contagem de c?lulas, IL-4, IL-5, IL-10, INF- ? e eotaxina (no lavado broncoalveolar) e realizada an?lise histol?gica do pulm?o. Foi observada significativa redu??o na contagem total de c?lulas nos grupos que foram imunizados com o extrato total sol?vel (ET) e com o extrato sol?vel dos sistemas (ES) de A. cantonensis, em rela??o ao grupo OVA (p<0,001). Verificamos uma redu??o significativa de eosin?filos, macr?fagos e linf?citos no grupo imunizado com ET, bem como nos grupos com ES, em rela??o ao grupo OVA. No entanto, a imuniza??o por via intraperitoneal com ET e ES n?o promoveu altera??o significativa nos n?veis de IgE espec?fica para OVA e citocinas avaliadas, exceto aumento de IL-10 e IFN- ? (p<0,01) no grupo imunizado com o ET. Concluindo, os diferentes extratos de vermes de A. cantonensis, em especial o ET, quando administrado precocemente por via intraperitoneal, resulta em uma modula??o na resposta pulmonar al?rgica induzida por OVA em camundongos, com uma resposta distinta de IL-10 e INF- ? no grupo ET.

Identiferoai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/7498
Date31 March 2017
CreatorsPascoal, Vanessa Fey
ContributorsPitrez, Paulo M?rcio Condessa
PublisherPontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Medicina/Pediatria e Sa?de da Crian?a, PUCRS, Brasil, Escola de Medicina
Source SetsIBICT Brazilian ETDs
LanguagePortuguese
Detected LanguageEnglish
Typeinfo:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis
Formatapplication/pdf
Sourcereponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS
Rightsinfo:eu-repo/semantics/openAccess
Relation557290555552975733, 600, 600, 600, -224747486637135387, -969369452308786627

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