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Previous issue date: 2017-04-25 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The aim of the present study was to asses the present of Bisphenol A in dental material used on CAD/CAM block.For this In Vitro study, blocks for CAD-CAM, Lava Ultimate, Enamic and Mark II in standardized fragments containeing Bisphenol A were evaluated. Ten fragments of each block were made: n = 30, immersed in deionized water on group 1 and artificial saliva Kin Hidrat on group 2 in a light protected bottle.Stored in a culture stove for 24 hours, all samples were put in 1,5ml bottles containing 1,0ml of artificial saliva (KIN HIDRAT, PharmaKIN, S?o Paulo, Brazil ) on group 1 and deionized water on group 2, and then freezed in -80?C for 24 hours. After the 24 hour period, the samples were submitted to High Efficiency Chromatography on Toxicology and Pharmacology Institute of PUCRS as
methodology described on this study.After cromatographic analysis of groups 1 and
2, the non-release of Bisphenol A was observed in all groups of samples tested. All
groups and samples presented peak release of unidentified substance of different
molecular weight and different breaking time than the Bisphenol A standard.
Additional researches using other analysis methods are needed to examine the
cumulative estrogenic effects of Bisphenol A dental materials for CAD CAM and
composite resins. / O objetivo geral deste estudo foi avaliar o componente Bisfenol A presente em blocos de materiais dent?rios utilizados no sistema CAD/CAM quanto a libera??o ou n?o de Bisfenol A. Foram avaliados para a realiza??o deste estudo in vitro, blocos para CAD/CAM, Lava Ultimate, Enamic e Mark II, em fragmentos padronizados, cuja matriz de resina composta continha Bisfenol A. Foram confeccionados 10 fragmentos de cada um dos blocos: n = 30, imerso em ?gua deionizada no grupo 1 e saliva artificial KinHidrat no grupo 2 em frasco protegido da luz. Armazenadas em estufa por 24hs,todas as amostras, ap?s o periodo de 24hs, foram armazenadas em recipientes de 1,5ml contendo 1,0 ml de saliva artificial (KIN HIDRAT,PharmaKIN,
S?o Paulo, Brasil ) no grupo 1 e ?gua deionizada no grupo 2 e ap?s congeladas ? - 80?C por 24hs. Decorrido o per?odo de 24h, as amostras foram submetidas a cromatografia de alta efici?ncia (HPLC) no Instituto de Toxicologia e Farmacologia da PUCRS com metodologia descrita no estudo.Ap?s an?lise cromatogr?fica dos
grupos 1 e 2 observou- se a n?o libera??o de Bisfenol A em nenhum dos grupos de amostras testadas.Todos os grupos e amostras apresentaram pico de libera??o de subst?ncia n?o identificada de diferente peso molecular e diferente tempo de quebra do padr?o de Bisfenol A. Pesquisas adicionais, empregando outros m?todos de an?lise s?o necess?rias para examinar os efeitos estrog?nicos cumulativos do Bisfenol A de materiais dent?rios para CAD/CAM e resinas compostas.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/8155 |
| Date | 25 April 2017 |
| Creators | Carvalho, Ligia Maria Nogarett Pibernat de |
| Contributors | Mota, Eduardo Gon?alves |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Odontologia, PUCRS, Brasil, Escola de Ci?ncias da Sa?de |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -8096554818733665164, 500, 500, 600, -2070498469879244349, 2075167498588264571 |
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