O presente trabalho descreve o isolamento e a identificação ou determinação estrutural de metabolitos secundários de folhas e raízes ou rizomas de espécies de aráceas, coletadas na Reserva de Picinguaba, no Estado de São Paulo. O material vegetal forneceu, após extração com hexano, etanol e etanol aquoso, os seus respectivos extratos. A composição de ácidos graxos presentes nos extratos hexânicos foram analisados por CG/EM, observando-se a predominância do ácido palmítico, tanto nas folhas, quanto nas raízes de todas as espécies estudadas. A partição dos extratos brutos com solventes orgânicos e o fracionamento cromatográfico de resíduos das fases resultantes permitiram o isolamento de fitil, fitol, palmitato de sitosterol, 3β-palmitoxil-colest-5-en-7-ona, colest-4-en-3-ona e coleste-4,22-dien-3-ona, a-tocoferol, sitosterol + estigmasterol, 3β-hidroxicolest-5-en-7-ona + 3β-hidroxi-coleste-5,22-dien-7-ona,p-hidroxibenzaldeído, ácido phidroxibenzóico, acilglicosil sitosterol, acilglicosil 3β-hidroxi-colest-5-en-7-ona, acilglicosil + 3β-hidroxi-coleste-5,22-dien-7-ona, acilglicosil 3β,8-dihidroxi-coleste-5-en-7-ona, e glicosil-sitosterol + glicosil-estigmasterol. A elucidação estrutural das substâncias isoladas foi baseada em métodos éspectrométricos, sobretudo na ressonância magnética nuclear de hidrogênio e de carbono 13. / The present work describes isolation and identification or structural elucidation of secondary metabolites from the Ieaves and roots or tubers of Araceae species which were collected in the Pinciguaba Reserve, São Paulo State, Brazil. The extraction of plant materiaIs with hexane, ethanoI, water-ethanol, yielded its respective extracts. The composition of fatty acids present in the hexane extracts was carried out on GC/MS and it was observed that palmitic acid was the major constituent in Ieaves and roots or tubers of all analysed species. The partition of the crude extracts with organic solvent and chromatographic fractionation of residues afforded phytyl, phytol, β-sitosteryl palmitate, 3β- palmitoxyl-cholest-5-en-7-one, choleste-4-en-3-one + choleste-4,22-dien-3-one, (1,- tocopherol, sitosterol + stigmasterol, 3β-hydroxy-cholest-S-en-7 -one + 3β-hydroxy-cholest-5,22-dien-7-one, p-hydroxybenzaldehyde, p-hydroxybenzoic acid, acylglucosyl sitosterol, acylglucosyl 3β-hidroxy-cholest-5-en-7-one, acylglucosyl 3β-hidroxy-cholest-5 ,22-dien-7-one, acylglucosyl 3β,8-dihydroxy-cholest-5-en-7 -one, 3β-O-β-D-glucopyranosylsitosterol + 3β-O-β-D-glucopyranosylstigmasteroI. The structural elucidation of isolated metabolites was performed by spectrometric techniques, mainly by 1H and 13C NMR.
| Identifer | oai:union.ndltd.org:IBICT/oai:teses.usp.br:tde-14082007-155130 |
| Date | 02 July 2007 |
| Creators | Ingrit Elida Collantes Díaz |
| Contributors | Massayoshi Yoshida, Edna Tomiko Myiake Kato, Norberto Peporine Lopes, Liliana Marzorati, Dulce Helena Siqueira Silva |
| Publisher | Universidade de São Paulo, Química, USP, BR |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | Spanish |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da USP, instname:Universidade de São Paulo, instacron:USP |
| Rights | info:eu-repo/semantics/openAccess |
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