Indiana University-Purdue University Indianapolis (IUPUI) / Dental biofilm is a main contributing factor in the initiation and progression of
dental caries. The maturation of dental biofilms is expected to alter the anti-caries
efficacy of fluoride compounds. In the first aim, we conducted a series of modeldevelopment
experiments to test different variables to standardize a reproducible in-vitro
microbial caries model. We evaluated: surface conditioning using saliva; sucrose
concentrations and caries lesion severity; growth media conditions and mineral
saturation; dental substrate types; pH cycling protocol characteristics. In the second aim,
we used the developed model to evaluate the changes in the anti-caries efficacy of three
fluoride compounds (Sodium fluoride (NaF); Stannous fluoride (SnF2); Amine fluoride
(AmF); and deionized water (DIW- negative control)) at increasing maturation of a
microcosm biofilm. We continued the pH cycling protocol for 4 days, 8 days, and 12
days. We tested biofilm cariogenicity and carious lesion severity at each maturation
stage. In the third aim, we used the developed model to test the effect of different
exposure periods (early vs. late exposure) of the biofilm to three fluoride compounds
(NaF, SnF2, AmF, DIW) in comparison to DIW. We also evaluated the recovery of
biofilm cariogenicity with each exposure period. We evaluated, for each exposure period
and recovery stage, biofilm cariogenicity and carious lesion severity. We analyzed the
relationships between different variables (biofilm age, fluoride compound type, exposure
period) using ANOVA models. In conclusion: 1. The present model allows testing the
effect of biofilm maturation on the anti-caries efficacy of fluoride compounds. 2. Biofilm maturation plays an important role in increasing biofilm tolerance against fluoride
treatment; it could also influence the selection of fluoride compounds to achieve optimum
cariostatic effect. 3. Exposure period, and type of fluoride compound, both influence the
biofilm tolerance to fluoride anti-caries effect; they may also result in a sustainable
release of fluoride over time. / 2021-08-21
| Identifer | oai:union.ndltd.org:IUPUI/oai:scholarworks.iupui.edu:1805/20539 |
| Date | 08 1900 |
| Creators | Ayoub, Hadeel Mohammed |
| Contributors | Lippert, Frank, Gregory, Richard L., Martinez Mier, E. Angeles, Anderson, Gregory |
| Source Sets | Indiana University-Purdue University Indianapolis |
| Language | en_US |
| Detected Language | English |
| Type | Dissertation |
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