Transfusion medicine is dependent upon the ability to hypothermically preserve red blood cell (RBC) function ex vivo. Recent evidence suggests that the deformability of hypothermically stored RBCs may be compromised, potentially leading to microvasculature occlusion and tissue hypoxia in transfused patients. The main objectives of this thesis were to develop an ektacytometric technique suitable for detecting RBC deformability changes, establish whether deficits in RBC deformability due to hypothermic storage are occurring, and investigate liposomes as a biopreservation tool to modify the function of RBC membranes. This thesis demonstrates that hypothermic storage leads to RBC deformability impairments which can be detected by ektacytometry. In addition, this thesis has shown that the membrane characteristics and deformability of hypothermically stored RBCs can be differentially modified by treatment with liposomes of varying chemical composition. Through these investigations, this thesis has contributed to the advancement of the fields of transfusion medicine and biopreservation science.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:AEU.10048/1898 |
| Date | 06 1900 |
| Creators | Stadnick, Hart Peter Argyle |
| Contributors | Dr. Jelena Holovati (Laboratory Medicine and Pathology), Dr. Jason Acker (Laboratory Medicine and Pathology), Dr. Rodrigo Onell (Laboratory Medicine and Pathology), Dr. Thomas Churchill (Surgery) |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Format | 2027465 bytes, application/pdf |
| Relation | Stadnick H, Onell R, Acker J P, Holovati JL. Eadie-Hofstee analysis of red blood cell deformability. Clinical Hemorheology and Microcirculation. 2010; 47(3) |
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