Resonant microcantilevers are promising transducers for bacteria detection because of their
high sensitivities. Surface stress and mass from adsorbates affect the resonant frequency.
We developed a novel method for decoupling the frequency contributions of a change in
mass and surface stress on a cantilever sensor validated in theoretical, finite element and
experimental framework.
Bacteria capture was achieved by several different chemical immobilization of T4
phages. The most successful bacteria capturing surface produced bacterial densities of
about 11 bacteria/100^m2. The developed theory is then applied to determine captured
bacterial mass on the cantilevers. This provides an estimate of the bacteria mass on
the cantilever. Two different functionalizations resulted in predicted bacterial densities
of 5 bacteria/100^m2 and 3 bacteria/100^m2. Poor densities relative to surface capture
experiments is caused by the boundary effects of the cantilever in solution. / Microelectromechanical Systems and Nanosystems
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:AEU.10048/430 |
| Date | 11 1900 |
| Creators | Glass, Nicholas |
| Contributors | Evoy, Stephane (Electrical and Computer Engineering), Brett, Michael (Electrical and Computer Engineering), Szymanski, Christine (Biological Sciences) |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Format | 8657488 bytes, application/pdf |
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