Anthocyanins have been shown to possess specific antioxidant capacities, which
may provide an underlying protective effect against many chronic diseases . Although the
antioxidant capacity of anthocyanins has been well established, less is known about the
extent to which specific anthocyanin composition affects total antioxidant capacity . The
aim of the present study was to compare the antioxidant capacity of two different soft
fruits, blackcurrant and grape, which have distinctly different anthocyanin profiles.
The anthocyanin profiles of grape and blackcurrant were characterized by
HPLC/MS coupled with a diode array detector. Results showed that blackcurrant
contained four predominant anthocyanins, cyanidin 3-glucoside, delphinidin 3-glucoside,
cyanidin 3-rutinoside, and delphinidin 3-rutinoside . In contrast, malvidin 3-glucoside,
delphinidin 3-glucoside, cyanidin 3-glucoside, petunidin 3-glucoside, and peonidin 3-
glucoside were the major anthocyanins found in grape . The concentration of individual
anthocyanins in all berries was quantified with HPLC/UV using cyanidin 3-glucoside as
an external standard . Finally, results showed a greater (p<0.05) antioxidant capacity of
blackcurrant compared to grape. The total antioxidant capacity of crude extracts from
each was measured by Oxygen Radical Absorbance Capacity (ORAC) and ABTS assays.
Anthocyanin antioxidant capacity index (AACI), derived from the product of
antioxidant (ORAC) activity for each of major anthocyanin present in blackcurrant and
grape, was also used to determine whether the antioxidant capacity of crude anthocyanin
fractions represents either the sum total anthocyanin content or, alternatively, a synergy
between different anthocyanins components . Our results indicated that a plausible
potential synergy between anthocyanin components in regards to ORAC antioxidant
capacity existed in blackcurrant and grape semi-purified anthocyanin extracts.
Furthermore, it could be concluded that both total anthocyanin content as well as
the composition of individual anthocyanins in soft fruits is important to assess total
antioxidant capacity of different berry sources .
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:BVAU.2429/912 |
| Date | 05 1900 |
| Creators | Hosseini-Beheshti, Elham |
| Publisher | University of British Columbia |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
Page generated in 0.002 seconds