Stem cell therapy is emerging as a possible method for treating many diseases and disorders, such as cardiovascular disease. In particular, stem cells may be able to revive the dead tissue caused by acute myocardial infarction (heart attack). Adipose-derived stem cells were labelled with 18F-fluorodeoxyglucose (FDG) and superparamagnetic iron oxide (SPIO) particles, for imaging with positron emission tomography (PET) and magnetic resonance imaging (MRI), respectively, and injected into several rats following induced myocardial infarction. Stem cell retention in the heart was investigated following three injection sites; two within the heart (intramyocardial and left intraventricular), and one easily accessible vein (tail vein). The PET and MR images were registered and the initial distributions analyzed using region of interest (ROI) analysis, to determine which injection method would result in the highest stem cell retention in the infarcted heart. The ROI results determined that the intramyocardial injection had the highest % injected dose (%ID) in the heart with 14 +/- 4%, followed by left intraventricular and tail vein with %IDs of 3.6 +/- 0.8% and 1.2 +/- 0.6%, respectively. The results indicate that stem cell delivery via intramyocardial injection should be utilized for optimal retention in the heart.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:MWU.1993/23302 |
| Date | 14 February 2014 |
| Creators | Dietz, Bryson |
| Contributors | Elhami, Esmat (Physics and Astronomy), Lin, Francis (Physics and Astronomy) Sherif, Sherif (Electrical and Computer Engineering) |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Detected Language | English |
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