Return to search

The subcellular localization and targeting pathway of hyaluronidase1

Hyaluronidases are endoglycosidases that catabolize hyaluronan, an abundant component of the extracellular matrix surrounding vertebrate cells. We characterized one of the hyaluronidases, HYAL1, an enzyme deficient in the lysosomal storage disorder Mucopolysaccharidosis IX. HYAL1 stably expressed in BHK cells resulted in several intracellular forms, but only one secreted form. Secretion was not increased by weak bases, and no phosphate was incorporated in metabolic labeling, suggesting this enzyme is not targeted to the lysosome by the mannose 6-phosphate dependent pathway. Further analysis revealed the various forms of HYAL1 differ only in glycosylation, and are all active at pH 3.8. The forms migrated in a Percol density gradient similarly to an endosomal marker, and with partial overlap with the lysosomal marker LPG120 (Lamp1). / May 2006

  1. http://hdl.handle.net/1993/233
Identiferoai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:MWU.anitoba.ca/dspace#1993/233
Date04 April 2006
CreatorsPatel, Nehal
ContributorsDr. Barbara Triggs-Raine (Biochem. and Med. Genetics) Dr. Steve Pind (Biochem. and Med. Genetics), Dr. Gilbert Arthur (Biochemistry and Medical Genetics) Dr. John Wilkins (Internal medicine/Immunology)
Source SetsLibrary and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada
Languageen_US
Detected LanguageEnglish
TypeElectronic Thesis or Dissertation
Format4980951 bytes, application/pdf

Page generated in 0.0022 seconds