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Improvement of canola protein gelation properties through enzymatic modification

The objective of this study was to improve canola protein gelation properties with the use of enzymes. Both cross-linking and limited proteolysis were explored. Enzyme treatments were performed prior to heat induced gelation. A texture analyzer, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and scanning electron microscopy were used to characterize the resulting networks. Enzymatic cross-linking with transglutaminase was shown to improve the gelation of canola protein isolate (CPI). To the contrary, proteolysis with trypsin, ficin and bromelin, did not enhance the gelation properties of CPI. / October 2006

  1. http://hdl.handle.net/1993/276
Identiferoai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:MWU.anitoba.ca/dspace#1993/276
Date12 September 2006
CreatorsPinterits, Alexandra
ContributorsArntfield, Susan (Food Science), Aluko, Rotimi (Human Nutritional Sciences), Fulcher, Gary (Food Science)
Source SetsLibrary and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada
Languageen_US
Detected LanguageEnglish
TypeElectronic Thesis or Dissertation
Format25066971 bytes, application/pdf

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