Bio-conversion of agricultural wastes provides a viable solution to multiple
environmental problems as well as production of natural products. Apple processing
for manufacturing juice, pies and sauce results in significant volumes of underutilized
by-products. This study aims to optimize the method for producing
fermentable sugars from apple processing by-products. The conditions required for
pre-treatment, polyphenol removal and enzymatic hydrolysis were optimized. The
optimized conditions for dilute sulfuric acid-based hydrothermal pre-treatment were
acid concentration of 1.5% (w/v) at 91 oC for 16 min. The final yield of 12.7%
fermentable sugars (glucose, fructose and galacturonic acid) was obtained after multistep
hydrolysis using commercial cellulase, pectinase and ?-glucosidase at 9, 38 and
8 enzyme units/g FW, respectively. The other optimum conditions were temperature
of 40 oC, pH at 4.0 and 24 h of reaction time. These fermentable sugars can further be
converted into bio-ethanol and organic acids using specific yeast and bacteria.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:NSHD.ca#10222/14354 |
| Date | 28 October 2011 |
| Creators | PARMAR, INDU |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
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