A PCR Differentiation Method, a hybrid of the US EPA and MOE methods, for quantifying human infectious C. parvum/C. hominis as a group and non-human infectious C. andersoni/C. muris was developed. Primers and probe sets targeting the hsp70 gene were designed for C. andersoni/C. muris; those for C. parvum/C. hominis were obtained from the MOE method. Results showed that C, andersoni/C. muris primers were specific for C. andersoni/C. muris oocysts, while those for C. parvum/hominis primers detected C. parvum/hominis and C. meleagridis. All primers were then used to quantify oocysts from urban and agricultural environmental water samples in Kitchener/Waterloo. Human infectious Giardia lamblia was also incorporated into this study. C. parvum/C. hominis and Giardia lamblia were detected at urban and agricultural areas, whereas C. andersoni/C. muris was only detected at agricultural sites. The PCR Differentiation Method is a reliable method for quantifying Cryptosporidium and Giardia lamblia in environmental water samples. / Best in Science Program of the Ontario Ministry of the Environment, Natural Sciences and Engineering Council (NSERC) of Canada Discovery Grants
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:OGU.10214/7443 |
| Date | 30 August 2013 |
| Creators | Duggal, Megha |
| Contributors | Lee, Hung, Trevors, Jack T, Weir, Susan |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Rights | Attribution-NoDerivs 2.5 Canada, http://creativecommons.org/licenses/by-nd/2.5/ca/ |
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