Immunomodulatory effects of lactic acid bacteria vary with strain and may
vary with growth phase and medium. The ability of different lactobacilli
strains (Lactobacillus helveticus R0052, L. rhamnosus R0011, L.
rhamnosus GG) at different growth phases to modulate macrophage and
intestinal epithelial cell cytokine production following a pro-inflammatory
challenge was examined. Modulation of cytokine production by human
macrophage cell lines (U-937) and intestinal epithelial cells (HT-29) induced
by Tumor Necrosis Factor α was assayed by ELISA for interleukin-8 (IL-8).
Granulocyte-macrophage colony stimulating factor (GM-CSF) production
was assayed by ELISA in the HT-29 cell line. Strain-dependent differences
were observed in the ability of viable bacteria and spent de Mann-Rogosa-
Sharpe (MRS) broths from log versus stationary growth phase in HT-29 and
U-937 cells. Overall, variation in the immunomodulatory activity of these
lactic acid bacteria and spent broths reflects not only strain variation but
potentially also differences in growth phase and substrate. / UOIT
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:OOSHDU.10155/69 |
| Date | 01 September 2009 |
| Creators | Cooper, William |
| Contributors | Forrester, Sean, Strap, Janice |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Thesis |
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