We investigated the capacity of human umbilical cord perivascular cells (HUCPVCs)
to repair tendon damage in a collagenase tendon injury model in rats. Our hypothesis was
that HUCPVCs would be able to produce an organized extracellular matrix and facilitate
tissue regeneration in this rat model. HUCPVCs were injected, in collagenase, into the
Achilles tendon of nude rats. Harvested tendons showed the presence of HUCPVCs at the
site of injury, whose morphology changed from rounded to elongated over the post-injury
time of 30 days. Human genes for collagen type 1 and -actin were expressed at all time
points and there was also a signi cant increase in the tensile strength and sti ness values
at 30 day post-injury in the experimental group. We conclude that HUCPVCs facilitated
regeneration in our model, and thus may be a putative cell source for the treatment of
tendon and ligament injuries.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:OTU.1807/24564 |
| Date | 26 July 2010 |
| Creators | Emrani, Hamideh |
| Contributors | Davies, John Edward |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
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