Fcγ receptor-mediated phagocytosis is an active process requiring receptor clustering as a signal initiation event. The mechanisms controlling Fcγ receptor clustering are unknown, as are the parameters governing the receptor lateral mobility in the plasma membrane. This work investigated the lateral mobility of Fcγ receptor IIa in resting primary human macrophages using single-molecule tracking methodology. In the absence of ligands, the receptor was found to exist mostly as a monomeric species. Detailed receptor dynamics revealed the existence of two receptor populations: one that was mobile, the other confined. The actin cytoskeleton was shown to be important for receptor confinement but did not affect receptor diffusion. Such findings are
important in understanding the mechanisms for receptor clustering and signal initiation in phagocytosis.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:OTU.1807/24567 |
| Date | 26 July 2010 |
| Creators | Farkash, Yoav |
| Contributors | Grinstein, Sergio |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
Page generated in 0.002 seconds