Preliminary investigations were carried out in order to explore the potential of laser-stimulated capillary growth in a blood vessel-on-a-chip. To fulfill the project objective, a series of experiments in both directions of two photon
fluorescence imaging and laser-semitransparent materials interaction were performed. A purpose-built two-photon
fluorescence imaging resolution was tested by imaging 1 micron diameter fluorescent beads. Also, the potential of fluorescence imaging in the waveguide writing eld as well as the biological eld was studied. Further, for laser ablation on the mouse artery loaded in the microfluidic channel, the processing window was found such that the damage induced by femtosecond laser just a effects the artery, not the other interfaces of the microfluidic chip. At the end, the result of laser trepanning on the mouse artery wall combined with two photon fluorescence imaging was shown. These results will be useful for more advanced
biological study such as angiogenesis.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:OTU.1807/27344 |
| Date | 30 May 2011 |
| Creators | Karimelahi, Samira |
| Contributors | Herman, Peter R. |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
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