Human chromosome 13q14 has been identified as one of the hotspots of deletion in prostate cancer, multiple myeloma, and chronic lymphocytic leukemia. Chromosome Condensation 1-like (CHC1L) is an uncharacterized gene in this region. CHC1L is found within the smallest common region of loss of heterozygosity in prostate cancer, and its decreased expression is linked to pathogenesis and progression of both prostate cancer and multiple myeloma. In the present study, we have generated Chc1L gene knockout mice and demonstrated that loss of this gene increases tumorigenesis in two year old mice. Knockout and heterozygous mice are predisposed to development of Histiocytic Sarcoma and Histiocyte-Associated Lymphoma. Bone marrow and splenic cells from 8-12 week old knockout mice have elevated viability ex vivo. These data provide the first direct evidence that CHC1L is a tumor suppressor gene involved in suppression of histiocyte-rich neoplasms.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:OTU.1807/33541 |
| Date | 27 November 2012 |
| Creators | Spillane, David |
| Contributors | Wen, Xiao-Yan |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
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