Optical brain imaging is proven to be useful to understand brain function and morphology at cellular and network level. Different optical imaging modalities were developed over the years, with our group developing multi-modal simultaneous imaging using Vertical Cavity Surface Emitting Lasers (VCSELs). This thesis improves and demonstrates the applicability of the imaging system and adapts it to portable imaging. Specifically, it was found that using multiple exposures provide better flow measurements when compared to tracking measurements. An intrinsic parameter to monitor the state of the Blood Brain Barrier (BBB) was also discovered, proving more practical than previous fluorescence methods. We finally demonstrate initial results of imaging flow velocities and fluorescence in awake and moving rodents using VCSELs, achromatic doublets and a CMOS camera. Future work involves developing new prototypes of the portable system for imaging of disease states in awake animals and minimizing movement artefacts for oxygenation measurements.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:OTU.1807/42673 |
| Date | 20 November 2013 |
| Creators | Atchia, Yaaseen |
| Contributors | Levi, Ofer |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
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